Arid3c (NM_001252622) Mouse Untagged Clone

CAT#: MC227383

Arid3c (untagged) - Mouse AT rich interactive domain 3C (BRIGHT-like) (Arid3c), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid3c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227383 representing NM_001252622
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCTGCAGAGGCAGCAGGCAGCACGGCTGGCCCAAGGGGTGGGGCCCTTGGCCCCTCCACGCT
  TACCGCTTCCACAACCTCCTCTGCTTGGCGCCCGGACCCTACAGGCTCCTGAGGGGGCTATAGGGGTGGT
  TGGGGCTGAAGAAGAGGAGGGTGCTGAAGATGAGGAGGGAGAGACACCCTTAGCAGAAGAGGAGACAGCT
  GAGCAGAGCCATCCAGGAGCCCGATGTCCCAACTCACCTTCCAGCCAGTCTCCTGGAATCCAACCACATG
  AGTGGACCTACGAGGAACAGTTCAAGCAGCTGTATGAACTCGATGCGGACCCCAAGAGGAAGGAGTTTCT
  GGATGACCTATTTAGCTTCATGCAGAAGAGGGGTACGCCAGTGAACCGGGTACCCATCATGGCCAAACAG
  GTGCTGGATCTGTATGCGTTGTTTCGCTTGGTGACAGCCAAGGGAGGCCTGGTGGAAGTCATCAACCGAA
  AAGTGTGGCGAGAAGTCACACGCGGCCTCAGTTTGCCCACCACCATCACCTCTGCCGCCTTCACGCTCCG
  TACACAGTACATGAAGTATTTGTACCCATACGAGTGCGAGACACGGGCACTCAGTTCCCCAGGGGAGCTT
  CAGGCTGCCATAGACAGCAACAGGCGTGAGGGCCGCCGCCAGGCTTACACCGCGGTCCCGCTCTTCAACT
  TAGCAGGGCCCACACCTCGGGGCGCTCCTGGCCCCGCCTCGAGTCATGGGCCCGCCCCGACCGCGACCCC
  GAACTGCCCGGGTCCCACCCAGGGTTCTGCTTCTGGTTTACCAGCGCACGCTTGCGCTCAACTGAGCCCG
  AGCCCAGTAAAGAAAGAGGAGAGTGGAATCCCACCCCCTCGTCTGGCGCTACCTATGGGCTTGGCCTCGG
  AAGCTACAAGGGAGAAGCTGGCACCGGAAGAACCTCCGGAGAAGAGGGCTGTGCTAATGGGCCCCGTGGA
  CTCACCTCGACTGGGGGCACCCCCCAGTTTCCTGCCCCGTGGCAAGGCTCCCCTAAGGGGTATCCTCTTT
  GCTCGACGCCAACCTGTGCCAGCTTCCTTGGGTCCAACCAATCCTCCACCTCTACCCTCTACAGGGCCCC
  CTTCCAGTACCTTGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252622
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252622.1, NP_001239551.1
• RefSeq Size: 1661 bp
• RefSeq ORF: 1140 bp
• Locus ID: 550619
• Cytogenetics: 4 A5
• Gene Summary: This gene is a member of the ARID (AT-rich interaction domain) family of proteins. The ARID domain is a helix-turn-helix motif-based DNA-binding domain. ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.