Tm6sf2 (NM_001293795) Mouse Untagged Clone

CAT#: MC227373

Tm6sf2 (untagged) - Mouse transmembrane 6 superfamily member 2 (Tm6sf2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tm6sf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227373 representing NM_001293795
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATCCCGCCCCTAGCCGGCAGGACTGTGGCAATGTCATTCTGCGCCCTGCCAGTGTCCTACCTAC
  TCAATCAAGTTTCGGCGTTCTCACAGCCCCTGTTTGTGGTGCTGACGAGTGCTCTGATACTGGGTCTGCT
  CTTCCTGGCAGTCTATAGCCTATCTCATGGGGAGATCACCTACGACCCTCTCTATGCTGTCTTTGTGATC
  TTCTCCTTCACCTCGGTGGTGGACCTTGTCATTGCACTCCAAGAAGATGGCTACATGATGGGCTTCATGG
  ATTTCTACACCAAGGAGGGTGAGCCCTACCTACGCACAGCACATGGCATCTTCATCTGCTACTGGGATGG
  TACTGTGCACTACCTGCTCTATCTGACAATGGCTGGTGCCATCCGCAAAAGGAAAAGGTACCGGAACCTT
  GGGCTCTACTGGCTGGGATCCTTCGCCATGAGCATCCTGGTGTTTCTCCCGGGAAACATCCTTGGTAAAT
  ACAGTTCAGAGATGAGGCCTACCTTCTTCCTCGCCATCCTGTACATGCTGGTCCCATGCTGGGCCGGTGT
  GAGGATCTTCAACCAGTCCCGGGCACCAACCTCCTATACCCCTGACATGGTACAGGAAGAACAGAAGAAA
  AGTCTCTTCCAGCGTCCGGCTGACCTGGCACTCATAGTATACCTCATCTTTGCAGCGCTCTTCACTGTCT
  TCCGGGGTCTGGTGGTACTTGACTGCCCCACAGATGCCTGCTTCATCTATATTTACCAATATGAGCCATA
  CCTTCGGGACCCTGTGGCCTACCCAAAGTTGCAGATGCTGATGTATTTGTTCTATGCTCTACCATTCTAC
  TGCCTGGCTGCCTACGCCCTTGCCTTCCCTGGCTGCTCCTGGCTGCCGGACTGGGCCTTGGTATTTGCTG
  GAGCCATTGGCCAGGCGCAGTTCTCACACATGGGTGCCTCCATGCACATGCGCACACCCTTCACCTACCG
  CGTGCCAGAGGACACCTGGGCTACCTTCTTTCTGAGCAACCTGCTATTGGCACTGGGTCCCCACCTGCTG
  GCTTTCCGCTGCCTGTGGCGGCCCGCCTTCTTCTTGCATGCAGCGCTGCCATCTTCGCCACAGGACCAAG
  ACAAGAAGCAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293795
• Insert Size: 1137 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293795.1, NP_001280724.1
• RefSeq Size: 1446 bp
• RefSeq ORF: 1137 bp
• Locus ID: 107770
• Cytogenetics: 8 B3.3
• Gene Summary: Regulator of liver fat metabolism influencing triglyceride secretion and hepatic lipid droplet content. May function as sterol isomerase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.