Dpf3 (NM_001267625) Mouse Untagged Clone

CAT#: MC227372

Dpf3 (untagged) - Mouse D4, zinc and double PHD fingers, family 3 (Dpf3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dpf3
• Synonyms: 2810403B03Rik; 6530402L11Rik; BAF45C; C78788; cer-d4; CERD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227372 representing NM_001267625
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTGTCATTCACAACCCCCTGAAAGCGCTTGGGGACCAGTTCTACAAGGAAGCCATTGAGCACT
  GCCGGAGCTACAACTCGAGGCTGTGCGCAGAGCGGAGCGTGCGTCTCCCCTTCCTGGACTCGCAGACTGG
  GGTGGCTCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGCGGCCCAGGCCTCGCTCCGGGCCAG
  TTGTACACATACCCTGCCCGCTGCTGGCGCAAGAAGCGACGATTGCACCCACCAGAGGACCCAAAACTAC
  GACTCCTGGAAATCAAACCCGAAGTAGAACTGCCCCTGAAGAAAGATGGATTTACCTCTGAGAGTACCAC
  ACTGGAAGCCTTGCTTCGCGGCGAGGGAGTAGAGAAGAAGGTGGATGCCAGAGAAGAGGAAAGCATCCAG
  GAGATACAGAGGGTTTTGGAAAATGATGAAAACGTAGAAGAAGGGAATGAAGAGGAGGATTTGGAAGAAG
  ATGTTCCCAAGCGCAAGAACAGGACCAGAGGACGGGCTCGCGGCTCTGCAGGCGGAAGGAGGAGGCATGA
  TGCCGCCTCTCAGGAAGACCACGACAAACCCTACGTCTGCGACATCTGTGGCAAGCGCTACAAGAACCGG
  CCAGGACTCAGCTACCACTACGCTCATACTCACCTGGCCAGCGAGGAGGGAGACGAAGCCCAAGACCAGG
  AGACCCGATCCCCACCCAACCACAGAAATGAGAACCACAGACCCCAGAAAGGACCAGACGGGACAGTCAT
  TCCTAATAACTACTGTGACTTCTGCTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGAGGCCTGAAGAG
  CTGGTGTCCTGTGCAGACTGTGGACGCTCTGGTCATCCAACTTGCCTGCAGTTCACTCTGAACATGACTG
  AGGCAGTTAAGACCTACAAGTGGCAGTGCATAGAGTGTAAATCCTGTATCCTGTGTGGGACCTCGGAGAA
  CGACGACCAGCTACTCTTCTGTGATGACTGCGATCGTGGCTATCACATGTACTGTTTAAATCCCCCAGTG
  GCTGAGCCCCCAGAAGGAAGCTGGAGCTGCCATTTATGCTGGGAGCTGCTCAAAGAGAAAGCATCAGCCT
  TTGGCTGCCAGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267625
• Insert Size: 1137 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267625.1, NP_001254554.1
• RefSeq Size: 3155 bp
• RefSeq ORF: 1137 bp
• Locus ID: 70127
• UniProt ID: P58269
• Cytogenetics: 12 D1
• Gene Summary: Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development (By similarity). Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).