Tgfb1i1 (NM_001289552) Mouse Untagged Clone

CAT#: MC227365

Tgfb1i1 (untagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tgfb1i1
• Synonyms: ARA55; hic-5; Hic5; TSC-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227365 representing NM_001289552
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCAGCCACAGACAGGGTCTGGAGAATCTTCAGGAACCACTGGGGACAAGGATCATCTATACAG
  ATGAAATCATGTCTCAGTTCCCATCTAGTAAAATGGCTGAAGGGGAAGAGAAGGAGGACCAATCTGAAGA
  CAAGAGCTCACCCACTGTCCCTCCCAGCCCATTCCCTGCCCCCTCAAAGCCTTCAGCCACCTCCGCCACT
  CAGGAACTGGATAGACTGATGGCCTCGCTCTCTGACTTCCGTGTTCAGAACCATCTTCCAGCCTCAGGGC
  CACCTCAGCCTCCAGCAGCGAGCCCCACCCGTGAAGGATGCCCATCTCCACCAGGACAGACTAGCAAAGG
  CAGTCTGGACACCATGCTGGGCCTGCTGCAGTCTGACCTCAGCCGTCGTGGTGTCCCCACACAGGCCAAA
  GGCCTCTGTGGCTCCTGCAATAAACCTATAGCTGGGCAAGTGGTTACAGCCCTGGGCAGAGCCTGGCACC
  CGGAGCACTTCCTTTGCAGCGGTTGTTCCACAACCCTGGGAGGCAGCAGCTTCTTCGAGAAGGATGGGGC
  TCCCTTTTGCCCCGAGTGCTACTTTGAGCGCTTCTCCCCACGATGTGGCTTCTGTAACCAACCCATCCGA
  CACAAAATGGTTACCGCCTTGGGCACCCACTGGCACCCAGAACATTTCTGCTGCGTCAGCTGCGGAGAGC
  CTTTTGGAGAAGAGGGTTTCCACGAGCGGGAGGGTCGTCCGTACTGCCGGCGGGACTTCCTGCAGCTGTT
  CGCCCCACGCTGCCAGGGCTGCCAAGGCCCTATTTTGGATAACTACATCTCGGCACTCAGCGCGCTCTGG
  CACCCAGACTGCTTCGTGTGCAGGGAATGCCTTGCGCCCTTCTCCGGAGGCAGCTTTTTTGAGCACGAGG
  GTCGCCCTTTGTGTGAAAACCATTTCCATGCTCAGCGTGGTTCGCTGTGTGCCACGTGTGGTCTCCCAGT
  GACCGGCCGCTGTGTGTCCGCTCTGGGCCGACGCTTCCATCCAGACCACTTCACCTGCACATTCTGCCTG
  CGCCCACTCACCAAAGGCTCCTTCCAGGAGCGCGCCAGCAAGCCTTACTGCCAGCCTTGCTTCCTGAAGC
  TCTTCGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289552
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289552.1, NP_001276481.1
• RefSeq Size: 3761 bp
• RefSeq ORF: 1131 bp
• Locus ID: 21804
• UniProt ID: Q62219
• Cytogenetics: 7 F3
• Gene Summary: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate exon in the 5' region, resulting in translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a.