Kcnj15 (NM_001039056) Mouse Untagged Clone

CAT#: MC227358

Kcnj15 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 15 (Kcnj15), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj15
• Synonyms: 4930414N08Rik; AI182284; AI267127; IRKK; Kir4.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227358 representing NM_001039056
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCATTCACCTTGGCATGTCCAGTGCCCCACTGGTGAAGCATACCAACGGGGTTGGACTCAAGG
  CCCACAGACCCCGAGTCATGTCAAAGAGTGGGCACAGTAATGTGAGAATCGATAAGGTAGACGGAATCTA
  TTTACTCTACCTCCAGGACTTGTGGACAACCGTCATCGACATGAAGTGGCGATACAAGCTCACCCTATTT
  GCTGCCACCTTTGTGATGACCTGGTTTCTGTTTGGAGTGGTCTACTATGCCATAGCCTTTATTCATGGTG
  ACTTACAACTTGGGGAATCTAATTCCAACCACACACCCTGCATTATGAAAGTGGACTCTCTCACAGGAGC
  ATTCCTCTTTTCCTTGGAATCTCAGACAACCATTGGCTACGGGGTCCGTTCCATCACAGAGGAGTGTCCC
  CATGCTATCTTCCTCTTAGTCGCCCAACTGGTCATCACCACATTGATTGAGATCTTCATTACGGGGACCT
  TTCTGGCTAAAATTGCAAGACCCAAAAAGCGAGCCGAGACCATTAAGTTCAGCCACTGTGCTGTCATCAG
  CAAGCAGAATGGAAAGCTATGCCTGGTCATCCAGGTGGCCAACATGAGGAAGAGTCTCCTGATTCAGTGC
  CAGCTCTCTGGAAAACTCCTGCAGACACACGTCACCAAAGAGGGAGAACGCATTCTCCTCAACCAGGCCA
  CTGTCAAATTCCACGTGGACTCCTCTTCCGAGAGTCCCTTCCTCATCCTGCCCATGACCTTCTACCACGT
  GTTGGATGAGACAAGCCCCCTGCGGGACCTCACACCCCAAAACCTAAAGGAGAAGGAGTTTGAGCTGGTG
  GTACTTCTCAACGCCACGGTGGAGTCTACCAGCGCCGTCTGCCAGAGCCGAACGTCTTACATCCCGGAGG
  AGATCTACTGGGGCTTTGAGTTTGTGCCTGTGGTTTCTCTCTCCAAAAATGGAAAGTATGTGGCTGATTT
  CAGTCAATTTGAGCAGATCAGGAAGAGCCCGGATTGTACCTTCTACTGTGCCGATTCTGAGAAGCAGAAG
  CTTGAAGAACAGTACAGGCAAGAGGACCAGAGGGAGCGGGAGCTGAGGAGCCTCCTGCTACAGCAGAGCA
  ATGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039056
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039056.2, NP_001034145.1
• RefSeq Size: 5046 bp
• RefSeq ORF: 1128 bp
• Locus ID: 16516
• UniProt ID: O88932
• Cytogenetics: 16 55.86 cM
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3, 5, 7, 9 and 11 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.