Aifm2 (NM_178058) Mouse Untagged Clone

CAT#: MC227339

Aifm2 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aifm2
• Synonyms: 5430437E11Rik; Amid; D730001I10Rik; PRG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227339 representing NM_178058
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCCCAGGTCTCGGTGGATACAGGAGCCGTGCACGTGGTGATCGTGGGCGGGGGCTTCGGAGGGA
  TAGCGGCTGCCAGCCAGCTGCAGGCGCTGAATGTCCCCTTCATGCTGGTGGATATGAAGGACTCCTTCCA
  CCACAATGTGGCAGCCCTCCGGGCCTCCGTGGAGAGCGGGTTCGCCAAAAAGACATTCATTTCGTACTCT
  GCGACCTTCAAGGACAACTTCCGCCAGGGCAAAGTGATTGGCATAGACTTGAAGAACCGGATGGTGTTGC
  TACAGGGTGGCGAGGCCTTGCCCTTCTCACATCTTATCCTGGCCACAGGCAGCACCGGACCCTTCCCTGG
  CAAGTTTAACGAGGTGTCCTGCCAGCAGGCAGCCATCCAGGCCTATGAGGACATGGTGAAGCAGATCCAG
  CGCTCACAATTCATCGTGGTGGTGGGAGGCGGCTCTGCAGGAGTAGAGATGGCAGCAGAGATTAAAACCG
  AGTACCCTGAGAAGGAGGTCACTCTTATCCACTCCAGAGTACCCCTGGCCGACAAGGAACTCCTGCCCTG
  TGTGCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGTGTGCAGCTGCTGCTGAGTGAGCGGGTGAGC
  AACCTGGAGGAACTGCCTCGCAATGAGTATCGGGAGTACATCAAGGTGGAGACAGACAAGGGCACGGAGG
  TGGCCACCAACATGGTGATTGTGTGCAATGGGATCAAGATCAACAGCTCTGCCTACCGCAGTGCATTTGA
  GAGTAGGCTGGCTAGCAATGGTGCTCTGAAAGTGAACGAGTTCCTCCAGGTGGAAGGTTACAGCAATATT
  TATGCCATTGGTGACTGTGCCGATACCAAGGAGCCCAAGATGGCCTACCACGCTGGCCTGCATGCCAATG
  TTGCCGTGGCCAACATCGTCAACTCCATGAAGCAGAGGCCACTCAAAGCTTACAAGCCAGGTGCGCTGAC
  ATTCCTCCTGTCCATGGGCAGAAATGATGGCGTGGGTCAGATCAGTGGCTTCTACGTAGGCCGCCTCATG
  GTGCGGCTGGCCAAGAGCAGGGACCTTCTCATCTCCACAAGCTGGAAAACCATGCGGCAGTCTCCACCGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178058
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178058.4, NP_835159.1
• RefSeq Size: 1490 bp
• RefSeq ORF: 1122 bp
• Locus ID: 71361
• UniProt ID: Q8BUE4
• Cytogenetics: 10 B4
• Gene Summary: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death (By similarity). May contribute to genotoxin-induced growth arrest.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 3' UTR exon, compared to variant 4. Variants 2, 3 and 4 encode the same isoform (2).