P2ry1 (NM_001282016) Mouse Untagged Clone

CAT#: MC227336

P2ry1 (untagged) - Mouse purinergic receptor P2Y, G-protein coupled 1 (P2ry1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P2ry1
• Synonyms: P2Y1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227336 representing NM_001282016
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGGTGCCTTGGTCGGTTGTCCCCAACGGGACGGATGCTGCCTTTCTGGCGGGCCTGGGCTCGC
  TTTGGGGAAACAGTACTGTCGCCTCAACTGCAGCAGTTTCCTCTTCATTCCAATGTGCCCTGACCAAGAC
  CGGTTTCCAGTTCTACTACCTGCCGGCTGTCTACATTTTAGTGTTCATCATAGGCTTCCTAGGCAACAGC
  GTGGCTATCTGGATGTTCGTTTTCCACATGAAGCCTTGGAGCGGCATCTCCGTGTACATGTTCAATTTGG
  CTCTGGCTGACTTTTTATACGTGCTCACCCTACCAGCCCTCATCTTCTACTACTTCAACAAGACTGACTG
  GATCTTCGGGGATGCTATGTGCAAGCTGCAGAGATTCATCTTCCACGTAAATCTCTACGGTAGCATCTTG
  TTCCTCACCTGCATCAGCGCACACAGGTACAGTGGCGTGGTGTACCCTCTCAAGTCTCTGGGCAGGCTCA
  AGAAGAAGAATGCCATTTATGTCAGCGTGCTGGTGTGGCTCATTGTGGTGGTGGCCATCTCCCCTATTCT
  CTTCTACTCTGGCACTGGGACTCGGAAAAACAAAACTGTCACCTGCTATGACACCACGTCCAATGATTAC
  CTGCGAAGTTATTTCATCTACAGTATGTGCACGACTGTGGCCATGTTCTGCATCCCTTTGGTGCTGATCT
  TGGGCTGTTATGGATTAATTGTTAAAGCTTTGATTTACAATGACCTGGACAACTCTCCGCTCCGGAGGAA
  ATCCATTTACCTGGTGATAATTGTCCTGACGGTGTTTGCTGTGTCTTATATCCCTTTCCATGTGATGAAA
  ACGATGAATTTGCGAGCACGGTTGGATTTCCAGACCCCAGAAATGTGTGATTTCAACGACAGGGTTTATG
  CCACTTATCAGGTAACAAGAGGTCTAGCAAGTCTCAACAGCTGTGTGGACCCCATTCTTTATTTCTTGGC
  TGGAGATACATTCAGAAGGAGACTGTCCCGAGCCACCAGGAAAGCTTCCAGGAGGAGTGAGGCCAATTTA
  CAATCCAAGAGTGAAGAAATGACTCTCAATATTTTATCTGAGTTCAAGCAGAATGGAGACACGAGTTTGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282016
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282016.1, NP_001268945.1
• RefSeq Size: 3624 bp
• RefSeq ORF: 1122 bp
• Locus ID: 18441
• UniProt ID: P49650
• Cytogenetics: 3 E1
• Gene Summary: Receptor for extracellular adenine nucleotides such as ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.