B4galt2 (NM_001253381) Mouse Untagged Clone

CAT#: MC227320

B4galt2 (untagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 (B4galt2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: B4galt2
• Synonyms: Ggtb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227320 representing NM_001253381
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGACTGCTGGGGGGGACGCTGGAGCGGGTCTGCAAGGCTGTGCTCCTTCTCTGCCTGCTGCACT
  TCCTTGTGGCCGTCATCCTCTACTTTGACGTCTATGCCCAGCACCTGGCCTTTTTCAGCCGCTTCAGTAC
  CCGAAGCCCAGCCCATGCCCTCTACCCTGCAGCCAGCAGCAGTACCAACTGCTCTCGGCCCAACGCCACT
  GCCGCTAGCTCCGGGCTGCCTGAAGTGCCCAGTGCCAGGCCTGGTCCCACAGCTCCAGTCATTCCGCCCT
  GTCCTGACGTGCCGCCTGGTCTTGTGGGCCGAGTGGTCATCGAATTCACCTCACCCATGCCTCTGGAACG
  GGTGCAGAGGGAAAATCCAGGCGTGCTCCTGGGCGGCCGCTATTCTCCACCCGACTGCACCCCAGCCCAA
  ACGGTGGCAGTCATCATCCCCTTTAGACACCGGGAGCACCACCTACGCTATTGGCTCCACTATCTGCACC
  CCATGTTGAGGCGGCAGCGGCTGCGCTACGGTGTCTATGTCATCAACCAGCATGGCGAGGAGACCTTCAA
  CCGAGCCAAGCTGCTCAACGTGGGCTTCCTAGAGGCACTGAAGGAGGATGCTGCCTATGACTGCTTCATC
  TTCAGTGATGTGGACCTGGTCCCTATGGATGACCGCAATCTGTACCGTTGTGGTGACCAGCCCCGCCACT
  TTGCCATCGCCATGGACAAGTTTGGCTTCCGGTTGCCCTATGCTAGCTACTTTGGAGGTGTGTCGGGCCT
  GAGCAAGGCCCAGTTTCTGAGGATCAATGGCTTTCCCAACGAGTACTGGGGCTGGGGTGGCGAGGATGAT
  GACATCTTCAACCGGATCTCCCTGACTGGGATGAAGATCTCACGCCCGGATGTCCGGATTGGCCGCTACC
  GCATGATCAAGCACGACCGGGACAAACATAACGAGCCCAACCCTCAGAGGTTTAATAAGATTCAAAACAC
  AAAGATGAGCATGAAGTGGGACGGCATTGGATCCGTGCGGTACCGAGTCTTGGAAGTGTCTCGGCAACCG
  CTCTTCACCAACATCACAGTGGACATTGGACAGCCCATGTCGTGGCTCACTCAAGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253381
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253381.1, NP_001240310.1
• RefSeq Size: 2467 bp
• RefSeq ORF: 1110 bp
• Locus ID: 53418
• UniProt ID: Q9Z2Y2
• Cytogenetics: 4 D2.1
• Gene Summary: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer protein-coding transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.