Csnk1e (NM_001289899) Mouse Untagged Clone

CAT#: MC227318

Csnk1e (untagged) - Mouse casein kinase 1, epsilon (Csnk1e), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Csnk1e
• Synonyms: AI426939; AI551861; AW457082; CK1epsilon; CKIe; KC1epsilon; tau
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227318 representing NM_001289899
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCGTGTGGGAAATAAGTATCGCCTGGGCCGAAAGATCGGCAGTGGCTCCTTTGGAGACATCT
  ACCTGGGTGCCAACATTGCCTCTGGTGAGGAAGTAGCCATCAAGCTCGAATGTGTGAAGACGAAACATCC
  CCAGCTCCACATCGAGAGCAAGTTCTACAAGATGATGCAGGGCGGAGTGGGGATCCCGTCCATCAAGTGG
  TGCGGGGCTGAGGGAGACTATAACGTGATGGTCATGGAGCTGCTGGGGCCCAGCCTGGAGGACCTCTTCA
  ACTTCTGTTCCCGGAAGTTCAGCCTCAAGACGGTGCTGTTGCTGGCCGACCAGATGATCAGCCGCATCGA
  GTACATACACTCCAAGAACTTCATCCACCGGGATGTGAAGCCCGACAACTTCCTCATGGGCCTGGGGAAG
  AAAGGCAACCTGGTGTACATCATTGACTTCGGCCTGGCCAAGAAGTACCGCGATGCCCGCACACACCAGC
  ATATTCCCTACCGGGAAAACAAGAACCTGACCGGCACTGCCCGCTATGCCTCTATCAACACCCACCTGGG
  CATTGAGCAAAGCCGTCGAGATGACCTAGAGAGCTTGGGCTATGTGCTCATGTACTTCAACCTGGGCTCC
  CTGCCCTGGCAGGGCCTCAAAGCAGCCACCAAGCGTCAGAAGTACGAGCGGATTAGCGAGAAGAAGATGT
  CAACGCCAATCGAGGTCCTCTGCAAAGGCTACCCCTCCGAGTTCTCAACATACCTCAACTTCTGCCGCTC
  CCTGCGGTTCGATGATAAGCCTGACTACTCCTACCTGCGCCAGCTCTTCCGAAATCTCTTTCACCGGCAG
  GGTTTCTCCTACGACTACGTCTTCGACTGGAACATGCTCAAATTCGGGGCTTCCTCGAGCCAGGCTCAGC
  CCCGAGACAACGAAGCTCTTGCGCCACCCTGCCCCCGCCCTTGGCCTTGCGCTGGGCCCGCATACTCACC
  CACTTACTGGTGCCCGGCGCCCCTGGGCACCCAAGGACCCCCAGATAGGCCGGTGGAGGAGGAGGTGGAA
  CAACTGCCCCCTCAAAGATATTGGCCTGTGGTCTGGACTCCAGGGCCCCAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289899
• Insert Size: 1107 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289899.1, NP_001276828.1
• RefSeq Size: 3174 bp
• RefSeq ORF: 1107 bp
• Locus ID: 27373
• Cytogenetics: 15 E1
• Gene Summary: The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR and in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a.