Nelfe (NM_001045864) Mouse Untagged Clone

CAT#: MC227307

Nelfe (untagged) - Mouse negative elongation factor complex member E, Rdbp (Nelfe), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nelfe
• Synonyms: D17H6S45; NELF-E; RD; Rdbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227307 representing NM_001045864
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGTGATACCTCCGGGGCTGAGTGAAGAGGAAGAGGCACTGCAGAAGAAGTTCAACAAACTCAAGA
  AGAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAGAGCAGCAGCGGCCCAGCGAGCCAGGGCGGTGTCAA
  ACGCTCACTGTCAGAGCAGCCTGTGGTGGACACTGCCACAGCAACAGAACAGGCCAAACAACTGGTGAAG
  TCAGGAGCCATCAGTGCCATCAAGGCTGAAACTAAGAACTCAGGCTTCAAACGTTCTCGGACCCTGGAAG
  GGAAGTTAAAGGACCCAGAGAAGGGGCCTGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATGTCTGCTGA
  TGAGGACCTACAGGAGCCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAAAGCTTTGTGTCTTCTAGT
  GATCGGCTTCGGGAACTGGGACAAGATGGAGAAGAAGCAGAGGCCCCCGGGGCTGGTGATGGCCCACCTC
  GAGGCTTTGACTGGAGCTATGAAGAGCATGGTAGCGCCCGCTCCTCAGCCTCCCCTCCCCGAAGCCGCAG
  CAGGGACCGAAGTCATGACCGGAGCCGAGATAGGGATCGGGACAAAGAGAGAGACAGAGACCGAGATCGA
  GATCGAGACAGAGACCGGGATAAAGACAAAGACAGAGACCGGGATCGAGACAGAGACCGGGACCGAGATC
  GGGAACGAGACCGAGAGGGCCCTTTCCGCAGGTCAGACTCATTCCCCGAACGGAGGGCCCCTCGAAAGGG
  GAACACACTGTATGTGTATGGAGAGGACATGACGCCCACCCTCCTCCGCGGGGCCTTCTCTCCCTTTGGA
  AATATCATCGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTCACCTACGAGAAGATGGAGTCTG
  CAGATCAGGCTGTTGCTGAGCTCAATGGGACCCAGGTGGAATCTGTGCAGCTTAAAGTCAACATAGCCCG
  AAAACAGCCCATGCTGGACGCTGCTACTGGCAAATCTGTCTGGGGCTCCCTTGCTGTCCAGAACAGCCCT
  AAGGGTTGCCATCGAGACAAGAGGACCCAGATTGTGTACAGTGACGATCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001045864
• Insert Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001045864.1, NP_001039329.1
• RefSeq Size: 1621 bp
• RefSeq ORF: 1104 bp
• Locus ID: 27632
• UniProt ID: P19426
• Cytogenetics: 17 18.4 cM
• Gene Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same protein.