Rad23a (NM_001297606) Mouse Untagged Clone

CAT#: MC227289

Rad23a (untagged) - Mouse RAD23a homolog (S. cerevisiae) (Rad23a), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad23a
• Synonyms: 2310040P19Rik; AL024030; HR23A; mHR23A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227289 representing NM_001297606
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCACCATCACCCTTAAAACGTTGCAGCAGCAGACCTTCAAGATCCGCATGGAACCTGACGAGA
  CGGTAAAGGTGCTGAAGGAGAAGATAGAAGCTGAGAAGGGTAGAGATGCTTTCCCCGTGGCTGGACAGAA
  ACTCATCTATGCTGGCAAGATCTTGAGTGACGATGTTCCCATCAAGGAATACCATATAGATGAGAAGAAC
  TTTGTGGTTGTCATGGTGACCAAGGCCAAAGCTGGCCAGGGTATCCCGGCACCCCCAGAGGCCTCACCCA
  CTGCTGTCCCGGAGCCCTCCACACCTTTCCCTCCAGTCCTGGCATCAGGCATGTCTCATCCCCCACCTAC
  CAGCAGAGAGGACAAGAGCCCATCAGAGGAGTCAACCACCACAACATCTCCAGAATCCATTTCTGGCTCT
  GTTCCCTCTTCAGGTAGCAGCGGGCGAGAGGAAGACGCAGCTTCCACATTAGTGACTGGCTCTGAATATG
  AGACGATGCTGACTGAGATCATGTCCATGGGCTACGAGCGGGAGCGGGTTGTGGCCGCATTGAGGGCCAG
  CTACAACAACCCCCACCGAGCTGTGGAGTACTTGCTCACGGGAATTCCAGGAAGCCCTGAGCCTGAACAT
  GGTTCTGTCCAGGAAAGCCAGGCGCCTGAGCAGCCGGCCACAGAAGCAGCAGGGGAGAACCCCCTGGAGT
  TCCTGCGGGACCAGCCTCAGTTCCAGAACATGCGGCAAGTGATTCAACAGAACCCAGCGCTCCTGCCTGC
  TCTGCTCCAGCAGCTGGGTCAGGAAAACCCTCAGCTCCTGCAGCAAATTAGCCGTCACCAGGAGCAGTTC
  ATCCAGATGTTGAATGAGCCTCCCGGGGAGCTGGCGGACATCTCTGATGTAGAGGGGGAGGTTGGTGCCA
  TAGGTGAGGAGGCCCCACAGATGAACTATATCCAGGTGACACCGCAGGAGAAGGAGGCTATAGAAAGGCT
  GAAGGCACTGGGCTTCCCAGAGAGCCTGGTGATCCAGGCCTACTTCGCGTGTGAAAAAAATGAGAATTTG
  GCTGCCAACTTCCTCCTGAGTCAGAACTTTGATGATGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297606
• Insert Size: 1092 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297606.1, NP_001284535.1
• RefSeq Size: 2101 bp
• RefSeq ORF: 1092 bp
• Locus ID: 19358
• UniProt ID: P54726
• Cytogenetics: 8 C2
• Gene Summary: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).