Mdm4 (NM_001302804) Mouse Untagged Clone

CAT#: MC227284

Mdm4 (untagged) - Mouse transformed mouse 3T3 cell double minute 4 (Mdm4), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdm4
• Synonyms: 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227284 representing NM_001302804
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGTGCAACAGAATACTCCAATCCCAGAAAAAGAACTG
  AAGAAGAGGATACTCACACACTGCCTACCTCACGACATAAATGCAGAGACTCCAGAGCAGATGAAGACTT
  GATAGAACATTTATCTCAAGATGAGACATCTAGGCTTGACCTTGATTTTGAGGAGTGGGACGTTGCTGGC
  CTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAACTGTATTCCTAAAAGTAATGGCTCAACTGATTTAC
  AGACAAATCAGGATATAGGTACTGCCATTGTTTCAGACACTACGGATGATTTGTGGTTTTTAAATGAGAC
  CGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGAAGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGG
  AAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAGGATGATGATCTTGAGGACTCCAGGTCCTTGAGCG
  ATGATACTGACGTGGAACTTACCTCTGAGGATGAGTGGCAGTGTACGGAATGCAAGAAGTTTAATTCTCC
  AAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAGAAAGGATTGGTATTCGGATTGTTCTAAATTAACT
  CATTCCCTATCTACATCTAATATTACTGCCATACCTGAAAAGAAGGACAATGAAGGAATTGATGTTCCCG
  ATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGCCTAAAGATGGATATTTAAAGGAGGAAAAGCCCAG
  GTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTTGGCTCATAGTTCTGAAAGCCAGGAGATCATCTCA
  AGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAGAAAGCTGAAACAGAAAGTATGGAAGATTTCCAGA
  ATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGCCTCGGGATGGGAACATTATTCATGGGAAGACGAG
  CCATCTGACGACATGTTTCCACTGTGCCAGGAGACTGAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAG
  AAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302804
• Insert Size: 1089 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302804.1, NP_001289733.1
• RefSeq Size: 7194 bp
• RefSeq ORF: 1089 bp
• Locus ID: 17248
• UniProt ID: O35618
• Cytogenetics: 1 57.75 cM
• Gene Summary: This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (5) uses an alternate splice site in an internal exon, compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform 1.