Bcat2 (NM_001243053) Mouse Untagged Clone

CAT#: MC227230

Bcat2 (untagged) - Mouse branched chain aminotransferase 2, mitochondrial (Bcat2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bcat2
• Synonyms: Bcat-2; Eca40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227230 representing NM_001243053
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAAGAACCACAGAAGAAGCCAGCCCCTAGCCAGGCACTGCTGTTTGGGAAGACCTTCACAGACC
  ACATGCTGATGGTGGAGTGGAATAACAAGGCTGGATGGGGCCCACCAAGGATCCAGCCCTTCCAGAACCT
  CACGCTACACCCAGCCTGCTCTGGTCTGCACTACTCTCTGCAGCTCTTTGAGGGCTTGAAAGCATACAAA
  GGTGGAGACCAGCAGGTACGCCTCTTCCGGCCCTGGCTCAACATGGACAGGATGCTACGTTCTGCTAGGC
  GCCTCTGCCTGCCAGACTTTGACAAGCAGGAGCTGTTGGAGTGTATCCGCCAGCTCATTGAAGTAGACAA
  AGACTGGGTTCCCGATGGCAACGGAACGAGCCTCTACGTGCGGCCCGTGCTGATCGGGAATGAGCCCTCG
  CTGGGTGTTGGTATGGTCACACAAGCCCTCCTGTACGTCATTCTGTGCCCTGTGGGCTCCTACTTCCCTG
  GAGACTCCATGACACCTGTCTCCCTCCTGGCGGACCCTTCATTCGTCAGAGCCTGGATAGGCGGAGTCGG
  TGACTGCAAGTTGGGGGGGAACTATGGACCCACTGTGGCTGTGCAGCGAGAAGCCCAGAAGAGAGGCTGC
  GAGCAGGTCCTCTGGCTGTACGGGCCAGACCACCAGCTCACTGAGGTGGGCACCATGAACATCTTTGTCT
  ACTGGACCCATGAAGACGGAGTACTGGAGCTGGTAACCCCCCCACTGAATGGCGTCATCTTGCCTGGAGT
  AGTTCGACAAAGTCTGCTGGATCTGGCCAGGACTTGGGGTGAGTTCCGGGTGGCAGAACGCAAGGTCACT
  ATGAAGGAATTGAAGCGGGCGCTGGAGGAAGGCCGAGTGCGAGAAGTCTTCGGCTCAGGCACTGCTTGTC
  AGGTCTGCCCGGTGCACCAAATCCTGTATGAAGGCAAGCAACTCCACATACCTACCATGGAGAACGGCCC
  AGAGCTCATCCTGCGCTTCCAGAAGGAACTGAAGGCTATTCAGTACGGAGCCAGTGCCCACGATTGGATG
  TTTCGGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243053
• Insert Size: 1062 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243053.1, NP_001229982.1
• RefSeq Size: 1692 bp
• RefSeq ORF: 1062 bp
• Locus ID: 12036
• Cytogenetics: 7 29.36 cM
• Gene Summary: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.[UniProtKB/Swiss-Prot Function]