Pbx3 (NM_001290576) Mouse Untagged Clone

CAT#: MC227221

Pbx3 (untagged) - Mouse pre B cell leukemia homeobox 3 (Pbx3), transcript variant b

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pbx3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227221 representing NM_001290576
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGATCAATCCAGGATGCTGCAGACTCTGGCCGGGGTGAACCTGGCCGGCCACTCGGTCCAGGGGG
  GTATGGCTCTGCCGCCTCCCCCTCACGGCCACGAAGGGGCGGACGGCGACGGCAGGAAGCAGGACATCGG
  CGACATCCTCCACCAGATCATGACCATCACCGACCAGAGCTTGGACGAGGCGCAAGCAAAGAAACATGCT
  CTGAACTGTCACAGAATGAAACCGGCGCTCTTCAGCGTCTTGTGTGAGATCAAAGAGAAAACAGGTCTCA
  GCATCAGAGGAGCCCAGGAGGAAGACCCTCCCGACCCCCAGCTAATGAGACTGGACAATATGCTTTTGGC
  AGAAGGGGTTTCAGGTCCTGAGAAAGGTGGGGGATCGGCGGCAGCAGCTGCAGCCGCGGCAGCCTCTGGA
  GGTTCTTCAGATAACTCTATTGAACACTCAGATTACAGAGCCAAATTGACCCAGATCAGACAAATCTATC
  ACACAGAACTGGAGAAATATGAACAGGCATGTAATGAATTCACAACACATGTGATGAACCTTCTCCGAGA
  ACAGAGTAGAACGCGTCCCATTTCCCCGAAAGAGATCGAAAGGATGGTGGGCATCATCCATCGGAAATTT
  AGTTCCATTCAGATGCAGCTCAAGCAAAGCACGTGTGAAGCAGTTATGATTTTGAGATCAAGGTTCCTTG
  ATGCCAGACGGAAAAGGCGTAACTTCAGTAAACAGGCTACGGAAATCTTGAATGAATATTTTTACTCACA
  CCTCAGCAACCCCTACCCCAGTGAAGAAGCCAAAGAGGAGCTGGCCAAGAAATGCAGCATCACAGTATCA
  CAGGTATCCAACTGGTTTGGCAACAAACGAATCAGGTACAAGAAGAACATCGGCAAATTTCAAGAGGAGG
  CCAATCTCTATGCTGCAAAAACAGCCGTGACAGCCGCACATGCAGTGGCTGCAGCCGTGCAGAACAACCA
  GACCAACTCACCCACCACGCCAAACTCTGGTGGATACCCTCCGTCATGTTATCAATCAGACGGGAGGCTA
  CAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001290576
• Insert Size: 1056 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290576.1, NP_001277505.1
• RefSeq Size: 2757 bp
• RefSeq ORF: 1056 bp
• Locus ID: 18516
• UniProt ID: O35317
• Cytogenetics: 2 22.84 cM
• Gene Summary: Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (b) lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant a. The encoded isoform (PBX3b) has a distinct C-terminus and is shorter than isoform PBX3a.