Crlf3 (NM_001277106) Mouse Untagged Clone

CAT#: MC227164

Crlf3 (untagged) - Mouse cytokine receptor-like factor 3 (Crlf3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crlf3
• Synonyms: BB164954; Creme; Creme9; cyto; Cytor4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227164 representing NM_001277106
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGCGCGATGGAGCCGGAGCCCGAGGTGCTGCTTCAGGAAGCCCGCGAGAACGTGGAGGCGGCGC
  AGAGCTACCGGCGGGAGCTGGGCCAGCGGCTGCAGGGGCTGCGGGAGGCGCAGAGGCAGATAAAGGAGAG
  TGCTTCACAGACAAGAGATGTCCTCAAACAGCATTTTAGTGATTTAAAAGGAACCCTTGGGAAGCTATTG
  GATGAGCGATTGGTGACCCTTTTGCAAGAGGTGGACACCATTGAACAGGAGACCATCAAACCACTAGATG
  ACTGCCAGAAACTCATAGAGCATGGGGTCAACACTGCAGATGACCTGGTCCGAGAAGGTGAGATTGCCAT
  TCTTGGTGGTATCGAAGAAGAGAGTGATAAACTGTGGAACTTTACCAAAAAGGCCTCACACATTCAGCTG
  GACAGCTTACCAGAAGTGCCCTTGCTAGTTGATGTACCCTGTTTGTCGGCTCAGTTGGACGATTCCATTC
  TTAACATAGTGAAAGACCACATTTTTAAGCATGGAACAGTAGCTTCCCGCCCACCAGTACAGATTGAAGA
  ATTAATAGAGAAACCTGGAGGCATCATAGTGCGATGGTGTAAGGTGGATGATGACTTCACAGCCCAAGAT
  TACAGGCTTCAGTTCCGCAAATGTACTGCAAATCATTTTGAAGACGTGTATGTAGGTTCTGAAACGGAGT
  TCATAGTGTTGCACATAGACCCCAATGTAGATTATCAATTCAGAGTCTGCGCCCGAGGAGACGGCAGGCA
  GGAATGGAGTCCTTGGAGTGTTCCCCAGACAGGCCACTCCACACTGGTGCCTCATGAGTGGACAACTGGC
  TTTGAGGGATACAGTCTGAGCAGTCGAAGAAATATAGCCCTCCGGAATGATGCCGAGTCCTCGGGTGTTC
  TCTACTCCAGCGCTCCAACCTACTTCTGTGGGCAGACGCTGACATTCAGGTGCAGTCTTTGTCAATGGAA
  AAGAAATGACCAATCAGTTGCCTGCAGTTACCTCTGGCTCCACTGTCACATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277106
• Insert Size: 1035 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277106.1, NP_001264035.1
• RefSeq Size: 2283 bp
• RefSeq ORF: 1035 bp
• Locus ID: 54394
• UniProt ID: Q9Z2L7
• Cytogenetics: 11 47.43 cM
• Gene Summary: This gene encodes a cytokine receptor-like factor that contains a fibronectin type 3 domain. The encoded protein may act as a negative regulator of the cell cycle. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.