Fut7 (NM_001289454) Mouse Untagged Clone

SKU
MC227144
Fut7 (untagged) - Mouse fucosyltransferase 7 (Fut7), transcript variant 5
  $503.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Fut7
Synonyms AI853193; FTVII; Fuc-TVII; FucT-VII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227144 representing NM_001289454
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTGTATTGGGTACCACCCCACCAGGAGGCTGCGGGCCTGGGGCGGCCTAGCTGGAGGAGCAACAT
TCATGGTAATTTGGTTTTTCTGGCTGTGGGGATCAGCTCCTGGAAGTGCCCCTGTGCCTCAGTCCACACT
CACCATCCTTATCTGGCACTGGCCTTTCACCAACCGGCCGCCAGAGCTACCTGGTGACACCTGCACTCGC
TATGGCATGGCCAGCTGCCGTCTGAGTGCTAACCGGAGCCTGCTAGCCAGTGCTGATGCTGTGGTCTTCC
ACCACCGTGAGCTGCAAACCCGGCAATCTCTCCTACCCCTGGACCAGAGGCCACACGGACAGCCTTGGGT
CTGGGCCTCCATGGAATCGCCCAGTAATACCCATGGTCTCCATCGCTTCCGGGGCATCTTCAACTGGGTG
CTGAGCTATCGGCGTGATTCAGATATCTTTGTACCCTACGGTCGCTTGGAGCCTCTCTCTGGGCCCACAT
CCCCACTACCGGCCAAAAGCAGGATGGCTGCCTGGGTGATCAGCAATTTCCAGGAGCGGCAGCAGCGTGC
AAAGCTGTACCGGCAGCTGGCCCCTCATCTGCAGGTGGATGTGTTCGGTCGCGCCAGCGGACGGCCCCTA
TGCGCTAATTGTCTGCTGCCCACTTTGGCCCGGTACCGCTTCTACCTGGCCTTTGAGAACTCACAGCATC
GGGACTACATCACTGAGAAGTTCTGGCGCAATGCCCTGGCGGCTGGTGCTGTACCCGTGGCGCTGGGACC
TCCTCGGGCCACCTACGAGGCTTTTGTGCCACCAGATGCCTTTGTACACGTGGACGACTTCAGCTCTGCC
CGTGAACTGGCTGTCTTCCTCGTCAGCATGAATGAGAGTCGTTATCGTGGCTTCTTTGCTTGGCGAGACC
GGCTCCGTGTGCGGCTCCTGGGTGACTGGAGGGAGCGCTTCTGCACCATCTGTGCCCGCTACCCTTACTT
GCCCCGCAGCCAGGTCTATGAAGACCTTGAAAGCTGGTTCCAGGCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001289454
Insert Size 1029 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001289454.1, NP_001276383.1
RefSeq Size 1864 bp
RefSeq ORF 1029 bp
Locus ID 14347
UniProt ID Q11131
Cytogenetics 2 A3
Summary Catalyzes alpha-1,3 glycosidic linkages involved in the expression of sialyl Lewis X antigens.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 3. Variants 1, 2, 4, 5, 6 and 7 encode the same isoform (a), which is shorter and has a distinct N-terminus, compared to isoform b.
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Documents
Resources
"NM_001289454" in other vectors (1)
SKU Description Size Price
MR229355 Fut7 (myc-DDK-tagged) - Mouse fucosyltransferase 7 (Fut7), transcript variant 5 10 ug
$289.00 $457.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.