Mgme1 (NM_001289630) Mouse Untagged Clone

CAT#: MC227122

Mgme1 (untagged) - Mouse mitochondrial genome maintainance exonuclease 1 (Mgme1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mgme1
• Synonyms: 8430406I07Rik; AI426476
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227122 representing NM_001289630
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTGCCTCTGACCTTCTGCAGGCTGCTTAGCAGGTTAAACAGATTTTCTGTAAAAGCAAGCCCTC
  CTGTGAGTTTCTCCACTTTCTCTTATTTGTGTAGCCAGAAGAAGAAAAACTCTTACGAAGCAGTAGACCA
  AGCGAAGTACTCTCGTTTAGTACGCTCTGTCTTGTCCAGAGGCCCGGCCCAGACTCCAGAGTCGTTGTTC
  AAGGAAGATGATGTACTGTATGGACCAGTGAGTAAGCATAAGGCTGCAGAGCCGGAGCCACAGGCCAGAG
  TCCCACAACACTGCTTTCCTATCTTCAATGAAGAGAGAACCGGGAAACCACACACAGATGCTTCTTCAAG
  CCCTTTGAAGATCCCTTTGCAAAGGAACTCGATACCCAGTGTGACCCGCATCCTTCAGCAGACCATGCCA
  CCTGAACAGAGCTTCTTTTTGGAGAGGTGGAAAGAGCGGATGGTTCTGGAGCTGGGAGAAGACGGGTTTG
  CAGAATATACTTCAAATGTGTTTTTACAAGGCAAACAGTTCCATAAAGCCTTGGAAAGCATACTGTCACC
  CCAGGAGAACCTAACAGGGGGAGAAGAGCACCCCCAGTGTGGCTACATCGAAAGCATCCAGCATATTCTG
  ACAGAAATCAGTGGTGTGCAAGCTCTGGAGAGTGCCGTCCAGCATGAGGCCTTGAAGTATGTAGGGCTGC
  TGGACTGTGTGGCTGAGTACCGGGGCAAGCTGTGTGTGATTGATTGGAAGACATCAGAAAAACCAAAACC
  TTTAATTCGAAATACATATGACAACCCGCTGCAAGTTGTGGCGTACATGGGTGCCGTAAACCATGATGCC
  CACTACAGTTTTCAGGTTCAGTGTGGATTAATTGTGGTGGCCTATAAGGATGGGTCCCCTGCGCACCCTC
  ACTTCATGGATGAAGAGCTCTGTTCCAAGTATTGGGCCAAGTGGCTTCTCCGACTAGAAGAATATACAGA
  AAAGCAAAAGAACCTGAGCGCTCCAGAGCCAGCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289630
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289630.1, NP_001276559.1
• RefSeq Size: 3107 bp
• RefSeq ORF: 1017 bp
• Locus ID: 74528
• UniProt ID: Q9CXC3
• Cytogenetics: 2 G1
• Gene Summary: Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. All four variants encode the same protein.