Gm21992 (NM_001290128) Mouse Untagged Clone

CAT#: MC227103

Rbm14 (untagged) - Mouse Rbm14-Rbm4 readthrough (Rbm14-rbm4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gm21992
• Synonyms: Rbm14-rbm4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227103 representing NM_001290128
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTTTTGTGGGCAATGTCGATGGGGCGGATACGACACCGGAGGAGTTGGCAGCTCTCTTCGCGC
  CCTATGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTTGCCTTCGTGCACATGCGCGAGAACGCTGG
  CGCGGTGCGCGCCATCGAGGCCCTGCATGGCCACGAGCTGCGTCCAGGTCGCGCGCTCGTGGTGGAGATG
  TCGCGCCCGAGGCCCCTGAACACTTGGAAGATTTTCGTGGGCAATGTATCGGCTGCATGTACAAGTCAGG
  AATTGCGCAGCCTCTTCGAGCGCCGTGGACGCGTCATCGAGTGTGACGTGGTAAAAGGCAAACGAATGCA
  CGTGCAGTTGTCCACCAGCCGGCTTAGGACCGCGCCCGGGATGGGAGACCAGAGCGGCTGCTATCGGTGC
  GGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCAATAGATCGTTCGGGCCGCGTGGCAGACTTGACCGAGC
  AATATAATGAGCAATATGGAGCAGTGCGTACGCCTTACACCATGAGTTATGGGGATTCATTGTATTACAA
  CAACACGTACGGAGCGCTCGATGCCTACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAGTGGCA
  GCTGCAGCTGCCTCTGCGTATAGTAACTATGCAGAGCAGACTTTGTCTCAGCTGCCACAAGTCCAGAACA
  CAGCCATGGCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACAATAGACATCTGTTGCCGCCTTCAGG
  AGCTGCAGCAGCAGCAGCAGCTGCTGCTGCTTGTACTGCAGCTTCTACTTCATATTACGGGCGGGATCGG
  AGCCCCCTGCGTCGCGCTACAGGCCCAGTCCTCACTGTTGGAGAGGGCTACGGTTACGGGCATGACAGTG
  AGTTGTCCCAAGCTTCAGCAGCTGCTCGGAATTCCCTGTACGACATGGCCCGGTATGAGCGGGAGCAGTA
  TGCGGATCGGGCGCGGTACTCAGCCTTTTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001290128
• Insert Size: 1011 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290128.1, NP_001277057.1
• RefSeq Size: 2420 bp
• RefSeq ORF: 1011 bp
• Locus ID: 102902673
• Cytogenetics: 19
• Gene Summary: This locus represents naturally occurring readthrough transcription between the neighboring Rbm14 (RNA binding motif protein 14) and Rbm4 (RNA binding motif protein 4) genes. Alternative splicing results in multiple transcript variants, some of which encode fusion proteins that share sequence identity with each individual gene product. A slight modification was made to the summary, since not all variants are protein coding [provided by RefSeq, Jun 2018]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.