Nfyc (NM_008692) Mouse Untagged Clone

SKU
MC227100
Nfyc (untagged) - Mouse nuclear transcription factor-Y gamma (Nfyc), transcript variant 1
  $503.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nfyc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC227100 representing NM_008692
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACAGAAGGAGGGTTTGGCGGTACCAGCAGCAGTGATGCCCAGCAAAGCCTCCAGTCCTTCTGGC
CCAGAGTCATGGAAGAAATCCGAAACTTAACAGTGAAAGATTTCCGAGTACAGGAACTCCCACTGGCTCG
TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCCCCTGTGCTGTTTGCT
AAGGCAGCCCAGATTTTTATCACTGAGCTGACTCTTCGAGCCTGGATCCACACAGAGGATAACAAGCGTC
GTACTCTTCAGAGGAATGATATTGCCATGGCAATTACAAAATTTGATCAGTTCGACTTTCTCATCGATAT
TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGCCAGGAGGAGGTACGCCAGTCTGTGACTCCTGCGGAG
CCTGTCCAGTACTACTTCACGCTGGCTCAGCAGCCCACTGCTGTCCAGGTCCAGGGACAGCAGCAAGGCC
AGCAGACCACCAGTTCTACGACCACCATCCAGCCTGGACAGATCATCATTGCACAGCCTCAGCAGGGTCA
GACCACACCCGTGACCATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTACAGGCCCAACCTCAGGGT
CAGGCCCAGCAGACCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATTACCAACACAG
GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCTGGCCAGCTGCAATATATCCGATTAGCCCAGCCTGT
ATCAGGCACCCAAGTTGTCCAAGGACAGATCCAGACACTTGCTACCAATGCCCAACAGATCACACAGACA
GAGGTCCAGCAAGGACAGCAGCAGTTCAGCCAGTTCACAGATGGACAGCAGCTGTACCAGATCCAGCAAG
TCACCATGCCTGCAGGCCAAGACCTCGCCCAGCCCATGTTTATTCAGTCAGCCAATCAGCCCTCTGATGG
GCAGACCCCCCAGGTGACTGGAGACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_008692
Insert Size 1008 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008692.4, NP_032718.2
RefSeq Size 1990 bp
RefSeq ORF 1008 bp
Locus ID 18046
UniProt ID P70353
Cytogenetics 4 56.52 cM
Summary Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the llonger isoform (1). Variants 1, 2, 4, 5 and 6 encode the same isoform (1).
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Documents
Resources
"NM_008692" in other vectors (1)
SKU Description Size Price
MR229311 Nfyc (myc-DDK-tagged) - Mouse nuclear transcription factor-Y gamma (Nfyc), transcript variant 1 10 ug
$289.00 $300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.