Gpr12 (NM_001010941) Mouse Untagged Clone

CAT#: MC227093

Gpr12 (untagged) - Mouse G-protein coupled receptor 12 (Gpr12), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr12
• Synonyms: Gpcr01; Gpcr12; Gpcr20; Gpcr21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227093 representing NM_001010941
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGAAGACCCGAAGGTCAATTTAAGCGGGCTGCCTCGGGACTGTATAGATGCCGGTGCTCCAGAGA
  ACATCTCAGCCGCTGTCCCCTCCCAGGGCTCTGTTGCGGAGTCAGAACCCGAGCTCGTTGTCAACCCCTG
  GGACATTGTCTTGTGCAGCTCAGGAACCCTCATCTGCTGTGAAAATGCCGTTGTGGTCCTTATCATCTTC
  CACAGCCCCAGCCTGCGAGCCCCCATGTTCCTACTGATAGGCAGCCTGGCTCTTGCAGACCTGCTGGCTG
  GCCTGGGACTCATCATCAATTTTGTTTTTGCGTACCTGCTTCAGTCAGAAGCCACCAAGCTGGTCACCAT
  CGGACTCATTGTCGCCTCTTTCTCTGCCTCTGTCTGCAGTTTGCTGGCTATTACTGTGGACCGCTACCTC
  TCGCTATATTACGCCCTGACGTACCACTCCGAGAGGACCGTCACCTTTACCTATGTCATGCTAGTGATGC
  TCTGGGGAACCTCCATCTGCCTGGGGCTGCTGCCCGTCATGGGCTGGAACTGCTTGAGGGACGAGTCCAC
  CTGCAGCGTGGTCAGACCTCTCACTAAGAACAACGCTGCCATCCTCTCCATCTCCTTCCTCTTCATGTTT
  GCTCTGATGCTTCAGCTCTACATCCAGATTTGTAAGATTGTGATGAGGCACGCCCATCAGATAGCCCTGC
  AGCACCACTTCCTGGCTACATCGCACTATGTGACTACCCGGAAAGGGGTCTCGACCCTGGCTCTCATCCT
  AGGGACCTTTGCTGCCTGCTGGATGCCTTTCACCCTCTATTCCTTGATCGCCGATTACACCTACCCTTCG
  ATCTATACCTATGCCACCCTCCTGCCCGCCACCTACAATTCCATCATCAACCCTGTCATTTACGCTTTCA
  GAAACCAAGAGATCCAGAAAGCCCTCTGCCTCATTTGCTGTGGGTGCATCCCTTCCTCGCTGTCTCAGAG
  AGCTCGGTCTCCCAGCGATGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010941
• Insert Size: 1005 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010941.2, NP_001010941.1
• RefSeq Size: 2485 bp
• RefSeq ORF: 1005 bp
• Locus ID: 14738
• UniProt ID: P35412
• Cytogenetics: 5 G3
• Gene Summary: Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production (By similarity). Promotes neurite outgrowth and blocks myelin inhibition in neurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1-5 all encode the same isoform (a).