Kcnk2 (NM_001281848) Mouse Untagged Clone

CAT#: MC227064

Kcnk2 (untagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnk2
• Synonyms: A430027H14Rik; AI848635; TREK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227064 representing NM_001281848
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACAGTCTCCACGATTTTCCTGGTGGTCGTCCTCTACCTGATCATCGGAGCCACGGTGTTCAAG
  GCATTGGAGCAGCCTCAGGAGATTTCCCAGAGGACCACCATTGTGATCCAGAAGCAGACCTTCATAGCCC
  AGCATGCCTGCGTCAACTCCACCGAGCTGGACGAACTCATCCAGGATTTGGAAACATCTCCCCACGAACT
  GAAGGTGGAAAAATATTCTGCATCATCTATGCCTTGCTGGGAATTCCCCTCTTTGGCTTTCTACTGGCTG
  GGGTTGGTGATCAGCTAGGAACTATATTTGGAAAAGGAATTGCCAAAGTGGAAGACACATTTATTAAGTG
  GAATGTTAGTCAGACGAAGATTCGTATCATCTCCACCATCATCTTCATCCTGTTTGGCTGTGTCCTCTTT
  GTGGCTCTCCCTGCGGTCATATTCAAGCACATAGAAGGCTGGAGCGCCCTGGACGCTATCTATTTTGTGG
  TTATCACTCTGACGACCATTGGATTTGGAGACTACGTGGCAGGTGGATCAGACATTGAATATCTGGACTT
  CTACAAGCCTGTGGTGTGGTTCTGGATCCTCGTTGGGCTGGCCTACTTTGCAGCTGTTCTGAGCATGATT
  GGGGACTGGCTACGGGTGATCTCTAAGAAGACGAAGGAAGAGGTGGGAGAGTTCAGAGCGCATGCCGCTG
  AGTGGACAGCCAATGTCACGGCCGAGTTCAAGGAAACGAGGAGGCGGCTGAGCGTGGAGATCTACGACAA
  GTTCCAGCGTGCCACATCCGTGAAGCGGAAGCTCTCCGCAGAGCTGGCGGGCAACCACAACCAGGAACTG
  ACTCCGTGTAGGAGGACCCTGTCTGTGAACCACCTGACCAGCGAGAGGGAAGTCCTGCCTCCCTTGCTGA
  AGGCTGAGAGCATCTATCTGAACGGTCTGACACCACACTGTGCTGGTGAGGACATAGCTGTCATTGAGAA
  CATGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281848
• Insert Size: 990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281848.1, NP_001268777.1
• RefSeq Size: 3155 bp
• RefSeq ORF: 990 bp
• Locus ID: 16526
• Cytogenetics: 1 H6
• Gene Summary: Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and has multiple differences in the coding region. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1.