Upp2 (NM_001289660) Mouse Untagged Clone

CAT#: MC227040

Upp2 (untagged) - Mouse uridine phosphorylase 2 (Upp2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Upp2
• Synonyms: 1700124F02Rik; AI266885; UDRPASE2; UP2; UPASE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227040 representing NM_001289660
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCCCGCCGCCCCCGACCCCCGACCCTTCGGGCCGCCCGGGCTGTGGGCTGCTGCGAGCCGGGCT
  GGCGGGACGCGGGGGAGGCGCGCGGCACGGGGAGCGGCCAGAGTATGGCTTCCATTTTGCCTGCTTCTAA
  TAGATCCATGAGACCTGACAAGAATACATACGAGAGAAAACGGTCTGTGTATGTTAAGAATCCATACCTG
  GAAGGCATGGACGAAGACATTCTCTATCACTTGGATTTAGGAACCAAAACACACAACCTACCAGCAATGT
  TTGGGGATGTAAAGTTTGTCTGCGTTGGTGGGAGCCCGAACAGAATGAAGGCATTTGCACAGTTTATGCA
  CAAGGAACTCCGGTTGGAGGGAGATGGAGAAGACATTGAAGACATCTGTGCGGGGACAGACAGATACTGC
  ATGTTCAAAACAGGCCCCGTGCTCTCCGTCAGTCACGGGATGGGCATCCCCTCCATTTCCATTATGCTCC
  ACGAACTCATCAAATTACTCCACCATGCACACTGCTGTGATGTTACCATCATCAGAATTGGAACGTCAGG
  GGGAATTGGGATTGCACCAGGCTCCGTTGTAATAACAGATACAGCTGTAGACTCCTTCTTCAAGCCTCGA
  TTTGAGCAGGTCATCTTGGATAACGTGGTCACCCGAAGCACTGAGCTTGACAAAGAACTGGCTAATGATC
  TGTTCAACTGTAGCAGAGAAATCCCCAATGTTCCAACCCTCATCGGACACACGATGTGTACCTATGACTT
  TTATGAAGGCCAAGGTCGCCTAGATGGGGCGTTATGCTCCTTTTCGAGAGAGAAAAAGCTAGACTACTTG
  AAGAGAGCATACAGAGCTGGTGTCAGGAACATTGAAATGGAATCGACTGTGTTCGCAGCCATGTGTGGCC
  TGTGTGGCCTGAGAGTAGTTCCTATGTCAGCAAATAGTGTCCGGCTGAAAACCTGCAAACTACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289660
• Insert Size: 978 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289660.1, NP_001276589.1
• RefSeq Size: 2219 bp
• RefSeq ORF: 978 bp
• Locus ID: 76654
• Cytogenetics: 2 C1.1
• Gene Summary: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains alternate 5' and 3' terminal exons, initiates translation at an alternate start codon and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes variant 2, which is shorter and has distinct N- and C-termini, compared to isoform 1.