Lsp1 (NM_001271508) Mouse Untagged Clone

CAT#: MC227025

Lsp1 (untagged) - Mouse lymphocyte specific 1 (Lsp1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lsp1
• Synonyms: Lsp-1; p50; pp52; WP34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227025 representing NM_001271508
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGCCCCGCACTCCTGAGGAGAAATGCTAGCAAACGGGGCCTGGAGAAGCTGCTGAGGCTCACAA
  CTCAGTGGAGAGAAGAAGACGAGGAAGAGGCTGCCCGTGAGCAGCGTCAACGGGAGCGAGAGAGGCAGCT
  ACAGGACCAGGACAAAGATAAAGAAGATGATGGTGGCCATTCCCTGGAACAGCCAGGACAGCAGACACTC
  ATCAGCCTGAAGTCCTCTGAACTGGATGAAGATGAGGGTTTTGGTGACTGGTCCCAAAAGCCGGAGCCAC
  GGCAGCAATTCTGGGGGAATGAGGGGACTGCAGAAGGGACTGAACCCTCTCAAAGCGAGAGACCAGAGGA
  GAAACAAACAGAGGAGAGTTCTCACCAAGCCAAAGTCCACTTGGAGGAGTCAAACCTGAGCTACAGGGAG
  CCCGATCCAGAGGATGCTGTTGGGGGTTCTGGGGAGGCGGAAGAGGTCAGGACCCCCAGCCCTTTGGCCT
  TAGAAGACACCGTTGAGCTGAGTTCACCTCCCCTGAGCCCTACCACCAAACTGGCTGATAGGACCGAGTC
  CCTGAACCGCTCCATTAAAAAGAGCAACAGTGTGAAGAAGTCTCAACCAACCTTGCCCATTTCCACAATT
  GATGAACGCCTGCAGCAGTATACCCAGGCCACTGAGTCTTCTGGCCGAACTCCTAAGCTGTCCCGCCAGC
  CCTCCATAGAGCTGCCCAGCATGGCCGTAGCCAGTACCAAGACTCTTTGGGAAACAGGAGAAGTGCAGAG
  TCAGTCTGCTTCTAAGACACCCTCCTGCCAGGATATAGTAGCTGGAGACATGAGCAAGAAAAGTCTGTGG
  GAGCAGAAAGGAGGCTCCAAGATCTCATCCACCATCAAGAGCACCCCATCTGGAAAGCGGTACAAGTTCG
  TGGCCACTGGACATGGGAAGTACGAGAAAGTACTTGTGGATGAGGGCTCAGCACCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271508
• Insert Size: 969 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271508.1, NP_001258437.1
• RefSeq Size: 2079 bp
• RefSeq ORF: 969 bp
• Locus ID: 16985
• UniProt ID: P19973
• Cytogenetics: 7 87.93 cM
• Gene Summary: May play a role in mediating neutrophil activation and chemotaxis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate 5'-most exon, initiates translation at an alternate start codon and uses an alternate in-frame splice site in the coding region compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.