Vgll2 (NM_001300957) Mouse Untagged Clone

CAT#: MC227022

Vgll2 (untagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vgll2
• Synonyms: C130057C21Rik; Vgl; vgl-2; Vi; VIT; VITO-1; Vito1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227022 representing NM_001300957
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGTCTGGATGTTATGTACCAGGTCTACGGTCCCCCGCAGCCTTATTTCGCAGCCGCCTACACTC
  CCTACCACCAGAAACTAGCCTACTACTCAAAAATGCAGGAAGCTCAAGAGTGCGCCAGCCCTGGCAGCAG
  TGCCAGCGGGAGCTCCTCATTTTCCAACCCAACCCCAGCCAGTGTCAAGGAAGAGGAGGGCAGCCCAGAG
  AAAGAGCGCCCGCCCGAAGCTGAGTACATCAACTCCAGATGTGTCCTCTTCACCTACTTCCAGGGGGACA
  TCAGCTCTGTGGTGGACGAACATTTCAGTAGGGCCCTTAGCCACCCAAGCAGCTACACCCCAAGCTGTAC
  CAGCAGCAAAGCACACAGAAGCTCTGGACCCTGGAGAGAAGGCACCTTCCCGATGAGCCAGCGCAGCTTC
  CCCGCCTCCTTCTGGAACAGCGCGTACCAGGCGCCTGTGCCCGCGCCACTAGGCAGTCCTCTGGCCGCCG
  CACACTCGGAGCTGCCCTTTGCCACCGACCCCTACTCTCCCGCCACTCTGCACGGCCACCTGCACCAGGG
  CGCGGCCGACTGGCACCACGCGCACCCGCACCACGCGCACCCGCACCATCCCTATGCGCTGGGCGGCGCC
  CTGGGAGCACAAGCCTCTGCCTACCCGCGGCCAGCAGTGCACGAGGTCTACGCGCCCCACTTCGACCCGC
  GCTATGGGCCGCTGCTCATGCCCGCGGCCACTGGCCGCCCCGGCCGCCTGGCCCCTGCCTCGGCGCCGGC
  TCCCGGCAGCCCTCCCTGCGAGCTTGCGGCCAAGGGCGAGCCGGCGGGCAGCGCATGGGCTGCGCCCGGG
  GGACCCTTCGTGAGCCCCACGGGGGATGTGGCCCAGAGCCTGGGTCTCAGCGTGGACTCAGGTAAGCGGA
  GGAGGGAATGCAGTCTCCCCTCTGCCCCTCCGGCACTGTACCCGACTCTGGGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300957
• Insert Size: 966 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300957.1, NP_001287886.1
• RefSeq Size: 1664 bp
• RefSeq ORF: 966 bp
• Locus ID: 215031
• UniProt ID: Q8BGW8
• Cytogenetics: 10 B3
• Gene Summary: This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.