Pard6a (NM_001286345) Mouse Untagged Clone

CAT#: MC226988

Pard6a (untagged) - Mouse par-6 family cell polarity regulator alpha (Pard6a), transcript variant 5


  "NM_001286345" in other vectors (1)

Reconstitution Protocol

USD 330.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Pard6a
Synonyms 0710008C04Rik; 2610010A15Rik; Par-6; PAR-6A; Par6; PAR6alpha; Par6c; TAX40; Tip-40
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226988 representing NM_001286345
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGGCCGCAGAGGACTCCGGCGCGCAGTCCCGATAGCATCGTCGAGGTGAAGAGCAAATTTGACG
CCGAGTTCCGACGCTTTGCACTACCCCGCACTTCGGTGAGAGGCTTTCAGGAGTTCTCGCGATTGCTGTG
TGTGGTACACCAGATCCCTGGCCTGGACGTCCTGCTTGGCTATACGGATGCTCACGCAGAAGGTGACTCG
AGTGGCCTGGCTTTTGCCTCCAACTCTCTACAAAGGCGCAAGAAAGGGCTCCTGCTACGACCAGTGGCAC
CTCTGCGCACCAGGCCACCCTTGTTAATCAGCTTGCCCCAAGATTTCCGCCAGGTGTCTTCAGTTATAGA
TGTGGACCTACTACCTGAGACCCACCGACGAGTGAGGCTGCACAAACATGGTTCAGACCGTCCCCTGGGC
TTCTACATTCGAGATGGCATGAGTGTTCGCGTGGCTCCCCAGGGCCTGGAGCGGGTTCCAGGTATCTTCA
TCTCCCGCCTGGTACGTGGGGGCCTGGCTGAGAGTACAGGGCTGCTGGCGGTCAGTGATGAGATCCTTGA
GGTCAACGGCATTGAGGTGGCCGGGAAGACCTTGGACCAAGTGACGGACATGATGGTCGCCAACAGCCAC
AACCTCATCGTCACTGTCAAGCCTGCCAACCAGCGTAATAATGTGGTACGGGGGGCATCTGGGCGTCTGA
CAGGGCCTTCCTCTGTAGGGCCTGGGCCTACTGATCCTGACAGTGACGATGACAGCAGTGACCTGGTCAT
TGAGAATCGCCACCCTCCCTGTTCTAATGGGCTGTCTCAGGGGCCCCTGTGCTGGGACCTGCAACCTGGC
TGCCTACTTCCTGGTGCTGGCAGCTCTCTGCCCTCCTTGGATAGCAGAGAGCAAGCCAATTCTGGCTGGG
GGAATGGCATGCGAGGTGATGTTAGCGGATTCAGCCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286345
Insert Size 951 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001286345.1, NP_001273274.1
RefSeq Size 1194 bp
RefSeq ORF 951 bp
Locus ID 56513
Cytogenetics 8 D3
Gene Summary Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) uses an alternate in-frame splice site in the coding region, compared to variant 1. This encodes a shorter protein (isoform 4), compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.