Lgals8 (NM_001199043) Mouse Untagged Clone

CAT#: MC226987

Lgals8 (untagged) - Mouse lectin, galactose binding, soluble 8 (Lgals8), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lgals8
• Synonyms: 1200015E08Rik; AI326142; D13Ertd524e; Lgals-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226987 representing NM_001199043
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCCTTAAATAACCTACAAAATATCATCTATAACCCGATAATCCCCTATGTTGGCACCATTACTG
  AGCAATTGAAGCCTGGCTCTCTGATTGTAATCCGTGGGCATGTCCCTAAAGATTCAGAAAGATTCCAGGT
  TGACTTTCAGCTGGGCAACAGCCTGAAGCCAAGAGCAGACGTGGCCTTCCACTTTAACCCTCGGTTCAAA
  AGGTCTAGCTGCATTGTTTGTAACACACTGACACAGGAGAAGTGGGGCTGGGAGGAGATCACCTACGACA
  TGCCCTTCAGAAAAGAAAAGTCCTTTGAGATCGTGTTCATGGTGCTCAAGAACAAATTCCAGGTGGCTGT
  GAACGGAAGGCATGTTCTGCTGTACGCCCACAGGATCAGCCCGGAGCAGATCGACACAGTGGGCATCTAC
  GGCAAAGTGAACATCCACTCCATCGGGTTCAGATTCAGCTCGGATTTACAGAGTATGGAAACATCTGCTC
  TGGGACTGACACAGATAAACAGAGAGAATATACAAAAGCCAGGCAAGCTCCAGCTGAGCCTGCCATTTGA
  AGCAAGGTTGAATGCCTCCATGGGTCCTGGACGAACCGTTGTCATTAAAGGGGAAGTGAACACCAATGCC
  CGAAGCTTTAATGTTGACCTAGTGGCAGGAAAAACAAGGGATATCGCTCTGCACTTGAACCCACGCCTCA
  ATGTGAAAGCATTTGTAAGAAATTCCTTTCTTCAGGATGCCTGGGGAGAAGAGGAGAGAAATATTACCTG
  CTTCCCATTTAGTTCTGGGATGTACTTTGAGATGATAATCTACTGTGATGTCCGGGAATTCAAGGTTGCT
  ATAAATGGTGTGCACAGCCTGGAGTACAAACACAGATTTAAAGACCTAAGCAGTATTGATACACTATCAG
  TCGATGGTGATATCCGTTTGCTGGATGTAAGGAGCTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199043
• Insert Size: 951 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199043.1, NP_001185972.1
• RefSeq Size: 2996 bp
• RefSeq ORF: 951 bp
• Locus ID: 56048
• UniProt ID: Q9JL15
• Cytogenetics: 13 4.64 cM
• Gene Summary: Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 3. Variants 1 and 2 encode the same isoform (1), which is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.