Ptgr2 (NM_001252626) Mouse Untagged Clone

CAT#: MC226906

Ptgr2 (untagged) - Mouse prostaglandin reductase 2 (Ptgr2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptgr2
• Synonyms: 1810016I24Rik; 9130222H03Rik; 9630002F03Rik; AI838763; B830026H24Rik; PGR-2; PRG-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226906 representing NM_001252626
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCAACGCTGTAAGATGAACGAGGACACTGGCACTGACTACTTGGCACCGTGGCAGCTGGCGCAGG
  TGGCTGATGGTGGAGGAATTGGAGTTGTAGAGGAGAGCAAGCACCAGAAGTTGACTAAAGGCGATTTTGT
  GACTTCGTTTTACTGGCCCTGGCAAACTAAGGCAATTCTAGATGGGAATGGCCTTGAAAAGGTAGACCCA
  CAACTTGTAGATGGACACCTTTCATATTTTCTTGGGGCTATAGGTATGCCTGGCTTGACTTCCTTGATTG
  GGGTACAGGAGAAAGGCCATATATCTGCTGGATCTAATCAGACAATGGTTGTCAGTGGAGCAGCAGGCGC
  CTGTGGATCTTTGGCTGGGCAGATTGGCCACCTGCTTGGCTGTTCCAGAGTGGTGGGAATTTGTGGAACG
  CAGGAGAAATGTCTCTTTTTGACCTCAGAGCTGGGGTTTGATGCTGCAGTTAATTACAAAACAGGGAATG
  TGGCAGAGCAGCTGCGAGAAGCGTGCCCGGGCGGAGTGGATGTCTACTTTGACAATGTTGGAGGTGACAT
  CAGCAACGCGGTGATAAGTCAGATGAATGAGAACAGCCACATCATCCTGTGTGGTCAGATTTCTCAGTAC
  AGTAACGATGTGCCCTACCCTCCTCCACTGCCCCCTGCAGTAGAAGCCATCCGGAAGGAACGAAACATCA
  CAAGAGAGAGATTTACGGTATTAAATTATAAAGATAAATTTGAGCCTGGAATTCTACAGCTGAGTCAGTG
  GTTTAAAGAAGGAAAGCTAAAGGTCAAGGAGACCATGGCAAAGGGCTTGGAAAACATGGGAGTTGCATTC
  CAGTCCATGATGACAGGGGGCAACGTAGGGAAACAGATCGTCTGCATTTCAGAAGATTCTTCTCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252626
• Insert Size: 909 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252626.1, NP_001239555.1
• RefSeq Size: 3250 bp
• RefSeq ORF: 909 bp
• Locus ID: 77219
• UniProt ID: Q8VDQ1
• Cytogenetics: 12 D1
• Gene Summary: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an additional exon in the 5' region, which includes an alternate AUG start codon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1.