Nt5c3 (NM_001252374) Mouse Untagged Clone

SKU
MC226882
Nt5c3 (untagged) - Mouse 5'-nucleotidase, cytosolic III (Nt5c3), transcript variant 2
  $330.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nt5c3
Synonyms 1600024P05Rik; 2610206B05Rik; 3110004A18Rik; AI842776; cN-III; lupin; Nt5c3a; Nt5y; p36
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC226882 representing NM_001252374
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTAATCAGGAGTCTGCTGTCCATTTGAAAATGATGCCAGAATTTCAGAAAAGCTCAGTTCGTATCA
AGAACCCTACAAGAGTAGAAGAAATTATCTGTGGTCTCATTAAAGGAGGCGCTGCCAAACTTCAGATAAT
AACAGACTTTGACATGACTCTAAGTAGGTTTTCCTACAATGGGAAAAGATGCCCAACGTGTCATAATATC
ATTGACAACTGTAAGCTTGTTACAGATGAATGTCGAAGAAAGTTACTGCAGCTAAAGGAGCAGTATTATG
CTATTGAAGTTGATCCTGTTCTCACCGTGGAAGAGAAGTTCCCTTACATGGTAGAGTGGTATACCAAATC
TCACGGTTTGCTTATTGAACAAGGCATACCAAAAGCCAAACTTAAAGAGATTGTGGCTGACTCTGACGTT
ATGCTCAAGGAAGGATATGAGAATTTCTTCGGTAAACTTCAACAGCACGGTATCCCTGTGTTCATATTTT
CTGCTGGTATTGGTGATGTACTTGAAGAAGTTATCCGGCAGGCTGGAGTTTATCACTCAAACGTCAAAGT
AGTGTCAAACTTCATGGATTTTGATGAAAATGGAGTGCTGAAAGGATTCAAAGGAGAGTTAATCCACGTG
TTCAACAAACACGATGGTGCCCTGAAGAATACAGACTACTTCAGCCAGCTGAAAGACAACAGCAACATCA
TTCTGCTCGGGGACTCCCAAGGAGACCTAAGAATGGCCGATGGCGTAGCCAATGTTGAACACATTCTGAA
AATTGGTTATCTGAATGACAGAGTAGACGAGCTTTTAGAAAAATACATGGACTCTTATGATATTGTTTTA
GTAAAAGAAGAATCATTGGAGGTTGTCAACTCAATTTTACAGAAGACATTATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001252374
Insert Size 894 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001252374.1, NP_001239303.1
RefSeq Size 1530 bp
RefSeq ORF 894 bp
Locus ID 107569
UniProt ID Q9D020
Cytogenetics 6 B3
Summary Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001252374" in other vectors (1)
SKU Description Size Price
MR229093 Nt5c3 (myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III (Nt5c3), transcript variant 2 10 ug
$289.00 $300.00

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