Nit1 (NM_001242580) Mouse Untagged Clone
CAT#: MC226814
Nit1 (untagged) - Mouse nitrilase 1 (Nit1), transcript variant 2
"NM_001242580" in other vectors (1)
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Nit1 |
Synonyms | AI255805; ESTM30; W57327 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC226814 representing NM_001242580
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCATCAACTTCCTGGGAGCTGCCCCTGGTGGCTGTGTGCCAGGTAACATCAACACCAAACAAGC AAGAGAACTTTAAAACATGTGCTGAGTTGGTTCAAGAGGCTGCCAGACTGGGTGCTTGCCTGGCCTTTCT GCCTGAGGCATTTGACTTTATTGCACGAAACCCTGCCGAGACATTACTCCTGTCCGAACCACTGAATGGG GATCTTTTGGGCCAATATAGCCAGCTTGCCAGGGAATGTGGAATCTGGCTGTCCTTGGGCGGTTTCCACG AGCGTGGCCAAGACTGGGAGCAGAATCAGAAAATCTACAATTGTCATGTGCTTTTGAACAGCAAGGGATC AGTAGTGGCCAGTTACAGGAAGACACATCTGTGCGATGTAGAGATCCCAGGTCAGGGGCCGATGAGAGAA AGCAACTATACCAAGCCTGGAGGCACTCTTGAGCCACCTGTCAAGACACCGGCTGGCAAGGTTGGTCTAG CAATCTGTTATGACATGCGGTTCCCTGAACTTTCTTTGAAATTGGCTCAAGCTGGGGCAGAAATACTTAC TTATCCTTCAGCCTTTGGATCTGTTACAGGTCCAGCCCACTGGGAGGTGCTGCTGCGGGCCCGCGCCATT GAATCTCAGTGCTATGTAATAGCAGCAGCGCAGTGTGGACGCCACCATGAAACAAGAGCAAGTTATGGCC ATAGCATGGTGGTTGACCCGTGGGGCACAGTGGTGGCCCGCTGCTCCGAGGGACCAGGCCTCTGCCTTGC TCGAATTGATCTCCACTTTCTACAACAGATGCGCCAACACCTGCCTGTGTTCCAGCACCGCAGACCTGAC CTCTATGGCAGTCTGGGTCATCCACTCTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001242580 |
Insert Size | 873 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242580.1, NP_001229509.1 |
RefSeq Size | 1374 bp |
RefSeq ORF | 873 bp |
Locus ID | 27045 |
UniProt ID | Q8VDK1 |
Cytogenetics | 1 79.35 cM |
Gene Summary | Catalyzes the hydrolysation of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site at the 5' end compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (2) with a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229025 | Nit1 (myc-DDK-tagged) - Mouse nitrilase 1 (Nit1), transcript variant 2 |
USD 330.00 |
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