Ispd (NM_001289504) Mouse Untagged Clone

CAT#: MC226798

Ispd (untagged) - Mouse isoprenoid synthase domain containing (Ispd), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ispd
• Synonyms: 4930579E17Rik; AV040780
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226798 representing NM_001289504
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTGGGCCGTGCAGCAGGCCCGCTGAGCCTGGGCATTGCGTGAGCGGCCCCGCGGGCGCGGGCT
  CAGCGTTCCCGGAGTCCCCGTTGTCCGTCGCTGGGGCGGAGCCCGGGAACCGCCCTGGAACCGTGGCCGC
  CGTGCTGCCGGCTGGGGGTTGCGGGGAAAGGATGGGCGTCCGCACCCCGAAGCAGTTCTGCCGGGTTCTG
  GAAAGGCCGCTCATCAGCTACACTTTGCAGGCTATGGAGAGAGTATGCTGGATAAAGGACATTGTTGTGA
  CAGTGACAGGGGAGAACATGGAAGCAATGAGAAGTATCATCCAGAGGTATGGGCATAAGCGCATCTCACT
  AGCTGAGGCTGGAGCCACGCGCCACAGATCAATTTTCAATGGACTGAAAGCCCTGGCAGAAGATCAGCCA
  GACTGTAAACTCACTAAGCCAGAAGTGGTGATTATCCATGACGCCGTGAGACCTTTTGTTGAGGAAGATA
  TCCTCCTGAGAGTTGTCTTAGCAGCTAAGGAACATGGGGCAGCAGGAGCAATTCGACCTCTGGTGTCCAC
  TGTCATCAGTCCCTCTGCTGATGGTCACTTAGACCACTCACTGGACCGTGCCAAGCATAGGGCAAGCGAA
  ATGCCCCAGGCTTTTCTCTTTGATGTCATCTATGAAGCGTATCAGCAGTGTAGTGATTTTGACTTGGAAT
  TTGGAACAGAGTGCTTGCAGTTGGCTCTAAAATACTGTCACAGGAAAGCAAAACTTGTAGAAGGGCCCCC
  TGCCCTCTGGAAGGTGACCTACAAACAAGACCTGTGTGCAGCTGAAGCCATGATTAAAGGTGTGTTCAAC
  CTTGTGACTGTCAGCGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289504
• Insert Size: 861 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289504.1, NP_001276433.1
• RefSeq Size: 3246 bp
• RefSeq ORF: 861 bp
• Locus ID: 75847
• UniProt ID: Q5RJG7
• Cytogenetics: 12 A3
• Gene Summary: Cytidylyltransferase required for protein O-linked mannosylation (By similarity). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (By similarity). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (By similarity). Not Involved in dolichol production (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded protein (isoform 4) is shorter and has a distinct C-terminus, compared to isoform 1.