Ccnc (NM_001290422) Mouse Untagged Clone

CAT#: MC226767

Ccnc (untagged) - Mouse cyclin C (Ccnc), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccnc
• Synonyms: AI451004; AU020987; CG1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226767 representing NM_001290422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGAACTTCTGGCAGAGCTCCCACTATTTGCAGTGGATTTTGGATAAACAAGATCTGTTGAAAG
  AGCGCCAAAAGGACCTAAAGTTTCTCTCAGAAGAGGAGTATTGGAAGTTACAAATATTTTTTACAAATGT
  TATCCAAGCATTAGGTGAACATCTTAAATTAAGACAACAAGTTATTGCTACTGCTACAGTCTATTTCAAG
  AGATTCTATGCTAGGTATTCTCTGAAAAGTATAGATCCTGTATTAATGGCGCCTACATGTGTGTTTCTGG
  CATCCAAAGTAGAGGAATTTGGTGTTGTCTCAAATACAAGATTGATTGCTGCTACTACTTCTGTATTAAA
  AACTAGATTTTCATATGCTTTTCCAAAGGAATTTCCTTACAGGATGAATCATATACTAGAATGTGAATTT
  TACCTCTTAGAATTAATGGACTGTTGCTTGATAGTGTATCATCCTTATAGACCTTTGCTCCAGTATGTGC
  AGGACATGGGCCAGGAAGACGTGCTGCTTCCCCTTGCATGGAGGATAGTGAATGATACCTACAGGACGGA
  TCTCTGTCTGCTGTACCCTCCGTTCATGATCGCTTTAGCTTGCCTACATGTAGCCTGTGTCGTACAACAG
  AAAGATGCTAGACAGTGGTTTGCAGAACTTTCTGTGGATATGGAGAAGATTTTGGAAATAATCAGGGTTA
  TTTTAAAACTGTATGAGCAGTGGAAGAATTTTGATGAGAGAAAAGAGATGGCAACTATTCTTAGTAAGAT
  GCCGAAACCAAAACCACCTCCAAACAGAAGCTCCCTGAGTGACTCTCCACTAATGGCAGGGCCTGAAGCT
  GCAAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290422
• Insert Size: 849 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290422.1, NP_001277351.1
• RefSeq Size: 3485 bp
• RefSeq ORF: 849 bp
• Locus ID: 51813
• UniProt ID: Q62447
• Cytogenetics: 4 A3
• Gene Summary: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) contains an alternate exon in the 3' coding region, compared to variant 1. Variants 2 and 4 encode the same isoform (2), which is shorter by an amino acid and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.