Ubxn10 (NM_001285930) Mouse Untagged Clone

CAT#: MC226739

Ubxn10 (untagged) - Mouse UBX domain protein 10 (Ubxn10), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubxn10
• Synonyms: 5730509E04Rik; A830047D02; Ubxd3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226739 representing NM_001285930
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATAGAAGCCCCTGTGAACTTTGCACCACCTGAACGCAGCACTGTGGTCAGCACCGCAGGCGACA
  GCTCCACCTGGCAGCCCAGCTCACTCAGGATGCACGTTATCAGGCCCAAGTCTGCCAAGGGCCGGAAGCG
  GCCGAATTTGCACAGACCACAAGGCATGGGGGACGGCTCCCCCAGTGCACTATCCTCTTCACCTCCACCA
  CGGTCCTCCGGGTCTCCCAGCAACCAGAAACCAGGAGTCTGTGCAACGGTCTCAACATCTCAGGGAGCTC
  CTGACGAGATGCCGGAGTTGCTGCTGCAGCAGGCACCGACACGGACCGCCTCATCTCTCAACAGGTACCC
  GGTCCTCCCATCCATCAACAGGAGGAGCTTAGAGGTGGGAGCCGTGGACACTGTGGCCAGCAAGACCAGT
  TCCCTGCAGCTGAGCAGCGTCCAGGCCCTTTACCAAGAGGATTCAAGCCAGGAAGACTCCAGAACGCAGG
  TCTGTGCCTTAGAGAAGAAGTTCATCATCCGAACCAAAAGACAGAGCTCCTCCAGGGCCTCCAACATCGA
  GGAGCCGTCGGACGAAGAGCCAAGGCTGCTGCTGGCTGTCCGGTCACCCTCAGGCCAAAGGTTCGTGCGC
  TATTTCAGGCCCAGTGACGACCTCCAGACCGTCCTCGAGGTGGCCGAGCAGAAAAACAAAGCCACCTACC
  AACACTGCAGCATTGAGACGATGGAAGTGCCCAGGAGACGGTTCTCCGACCTCACCAAGTCCCTCCAGGA
  ATGCGGCATCCTTCATAAATCCGTGTTGGGCATCTCGCAGGAGGAGGGGGAGGCGTGGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285930
• Insert Size: 834 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285930.1, NP_001272859.1
• RefSeq Size: 3069 bp
• RefSeq ORF: 834 bp
• Locus ID: 212190
• UniProt ID: Q8BG34
• Cytogenetics: 4 D3
• Gene Summary: VCP/p97-binding protein required for ciliogenesis. Acts as a tethering factor that facilitates recruitment of VCP/p97 to the intraflagellar transport complex B (IFT-B) in cilia. UBX domain-containing proteins act as tethering factors for VCP/p97 and may specify substrate specificity of VCP/p97.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.