Mtif3 (NM_001256101) Mouse Untagged Clone

CAT#: MC226734

Mtif3 (untagged) - Mouse mitochondrial translational initiation factor 3 (Mtif3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mtif3
• Synonyms: 2810012L14Rik; AI414549
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226734 representing NM_001256101
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTTCTTCTGATGAGGTTGATGCTACAAACTACAAAGCTTGACCATAACTTAATTGGAAGATGTT
  TGCAAAGACACGCTGTGAAGCCAGATCCAGCACAGCTGTCCCTGTCTGCGTCCACCCCAAAGCTGTTGTA
  CCTGACTTCTGCAAAAGGTTTTAGTACTGCTGGAGACCCACAGGGTGAGAGGAAACAGAAAAGAAGGGAT
  GCTTTCTCTAACACCGGCCGGAAGATTAGTGAGCGGATTATCCGCGTCCTGGATGAGAAGGGCATGGACC
  TGGGGATGATGCATCGAGCAGATGTGATCAGACTCATGAACAAGCAGGACCTGAGGCTGGTGCAGAGGAA
  CACCAGCTCAGAGCCACCGGAGTACCAGCTTATGACCGGAGAGCAGATCCACCAGGAGCGGCTAAAGCTC
  CGGGAACAGGAAAAGGCCAAACCCAAAACTGGACCAACTATGACGAAAGAGCTCGTTTTCTCTTCAAATA
  TTGGACAGCACGATTTAGACACCAAGAGTAAACAGATCCAGCAGTGGATAGAGAAGAAATACCACGTTCA
  AGTCACGATAAAGAGGAGGAAAGATGCGGAGCAGTCAGAGGAGGAAACGGAGGAGATATTTAACCAGATT
  CTCCAGACTATGCCTGACATAGCAACCTTCTCAAGCAGACCAAAAGCCATCAGAGGAGGGACTGCTTCCA
  TGTGTGTTTTTCGTCACTTGAGCAAGAAAGAAGAAAAGGCGTACAGAGAATCCCAGGAGAGCCAGAGAAG
  AGACACTTTGAGCAAAGACGACGACGGAAACAGCAAAGAGTCGGATGTTGTGTGTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256101
• Insert Size: 831 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256101.1, NP_001243030.1
• RefSeq Size: 5128 bp
• RefSeq ORF: 831 bp
• Locus ID: 76366
• UniProt ID: Q9CZD5
• Cytogenetics: 5 G3
• Gene Summary: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All five variants encode the same protein.