Uros (NM_001302087) Mouse Untagged Clone

CAT#: MC226676

Uros (untagged) - Mouse uroporphyrinogen III synthase (Uros), transcript variant 4


  "NM_001302087" in other vectors (1)

Reconstitution Protocol

USD 330.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Uros
Synonyms AI415298; Ur; UROIIIS; Uros3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226676 representing NM_001302087
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTTCTCTTACTAAAAGACGCCAAGGAGGATGACAGCGGCCTGGATCCATATATCCAGGAGCTGC
GATTGTGTGGCCTAGAAGCCACACTGATTCCTGTGCTGTCATTTGAGTTTATGTCTCTCCCCAGTTTGTC
AGAAAAGCTGTCTCATCCTGAAGGCTTTGGAGGACTCATTTTCACCAGCCCCAGGGCAGTGGAAGCAGTG
AAGCTGTGTTTGGAGAAGGACAATAAAACTGAAGCCTGGGAGAAGTCTCTGAAAGACAGATGGAATGCCA
AGTCTGTGTACGTGGTTGGAAGTGCCACCGCTTCTCTAGTGAATAAAATTGGTCTGGATGCAGAAGGAGC
GGGCAGTGGAAATGCAGAAAAGCTTGCTGAATATATTTGCTCAAAGCCATCTTCAGAGCTGCCTCTTCTC
TTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGGATCCCCATGGAAA
GCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCA
GGGTATCCCAGCCAGCATCACGTTTTTCAGTCCCTCCGGCCTTAAATACAGCCTCGAGTATATTCAGGCG
TTATCTGGCAGCAGCTTTGACCAGATTAAGTTTATAGCCATTGGCCCCAGTACAACCCGTGCTATGGCTG
CTAAGGGCCTGCCTGTGAGCTGCACTGCAGAGAGCCCCACACCACAAGCCCTGGCTGCAGGCATCAGGAA
TGTGCTGAAGCCAAACCACTGTTGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302087
Insert Size 798 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001302087.1, NP_001289016.1
RefSeq Size 1546 bp
RefSeq ORF 798 bp
Locus ID 22276
UniProt ID P51163
Cytogenetics 7 77.26 cM
Gene Summary The protein encoded by this gene is the fourth enzyme in the heme biosynthesis pathway. It converts hydroxymethylbilane to uroporphyrinogen III, a cyclic tetrapyrrole. This enzyme is defective in the autosomal recessive disorder congenital erythropoietic porphyria. Alternate promoter usage controls cell type-specific expression, including erythroid cell-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Transcript Variant: This variant (4) differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, and 5 all encode the same isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.