Rdh13 (NM_001290411) Mouse Untagged Clone

CAT#: MC226661

Rdh13 (untagged) - Mouse retinol dehydrogenase 13 (all-trans and 9-cis) (Rdh13), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rdh13
• Synonyms: 8430425D21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226661 representing NM_001290411
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGTGCGAGGTGGCTGCAAAGGACATTCGAGGAGAGACCTTGAATCCCCGCGTACGTGCTGAAC
  GCCTAGACTTGGCTTCCCTCAAGTCCATCCGAGAGTTTGCAAGGAAGGTCATTAAAGAGGAGGAGCGAGT
  AGACATTCTGGTCAACAACGCAGCCGTGATGCGGTGCCCACACTGGACCACTGAGGATGGCTTTGAGATG
  CAGTTTGGTGTCAACTACTTGGGGCATTTTCTATTGACAAATTTGCTGTTGGACAAGCTGAAGGCCTCGG
  CCCCATCACGCATCATCAATCTCTCGTCCCTGGCTCACGTTGCTGGGCACATAGACTTCGAGGACTTGAA
  CTGGCAGATGAAAAAGTATGACACCAAGGCAGCCTACTGCCAGAGCAAGTTGGCTGTTGTCCTCTTCACC
  AAGGAGCTGAGCCACCGGCTGCAAGGCTCTGGTGTGACGGTCAATGCACTGCACCCTGGTGTGGCCAGGA
  CAGAGCTGGGGCGACATACGGGCATGCACAACTCTGCCTTCTCTGGCTTCATGCTTGGGCCCTTCTTCTG
  GCTGCTGTTCAAGAGTCCCCAGCTGGCGGCCCAGCCCAGCACATATCTGGCCGTGGCAGAAGAACTGGAG
  AATGTTTCGGGGAAGTACTTTGATGGACTCAGAGAGAAGGCTCCATCTCCTGAGGCTGAAGATGAGGAAG
  TAGCCCGCAGACTTTGGACGGAAAGTGCCCGTTTGGTGGGCTTGGCCATGGCTCATGGATCCCCTGGGAG
  AGGACATGCCATTCCCAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290411
• Insert Size: 792 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290411.1, NP_001277340.1
• RefSeq Size: 3077 bp
• RefSeq ORF: 792 bp
• Locus ID: 108841
• UniProt ID: Q8CEE7
• Cytogenetics: 7 A1
• Gene Summary: Retinol dehydrogenase with a clear preference for NADP. Oxidizes all-trans-retinol, but seems to reduce all-trans-retinal with much higher efficiency. Has no activity towards steroid.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice junction at the 3' end of the first exon and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.