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Tmem176b (NM_001286651) Mouse Untagged Clone

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SKU
MC226658
Tmem176b (untagged) - Mouse transmembrane protein 176B (Tmem176b), transcript variant 5
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $330.00
2 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tmem176b
Synonyms 1810009M01Rik; Clast1; Lr8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC226658 representing NM_001286651
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCAGAGCACAGTGACTGTGAATGGAGTCAAAGTGGCCTCCACACATCCCCAGTCCGCTCACATCA
GCATCCACATCCACCAAAAATCTGCTTTGGAGCAGCTGCTGGGAGCTGTGGGCTCCCTGAAGAAGTTTCT
CTCCTGGCCTCAGGCCAGAATCCACTATGGGCAGCTGTCTCTAGGGGTGACCCAGATATTGCTGGGGCTT
GTGAGCTGTGCTCTTGGAGTATGTCTCTACTTCGGGCCTTGGACTGAGCTGTGCGCCTTTGGCTGTGCTT
TCTGGTCGGGGTCTGTGGCAATTCTAGCTGGAGTTGGTACTATTGTCCATGAGAAGAGGCAGGGAAAACT
GTCCGGCCAGGTATCATGTCTCCTCCTCCTGGCTTGCATTGCCACAGCTGCAGCTGCTACCGTTCTGGGT
GTGAACAGCTTAATCAGGCAAACCAGTGTTCCCTACTACGTCGAGATCTTTTCCACATGCAACCCCTTGC
AATCAAGCATGGACCCTGGGTATGGGACAGTGCGATACAGTGATGATTCAGACTGGAAGACAGAGAGGTG
CAGAGAGTACCTGAACATGATGATGAACTTGTTCCTAGCGTTCTGCATCATGCTCACAGTGGTCTGTATC
CTGGAGATTGTTGTGTCTGTGGCTTCCCTGGGACTAAGTCTCCGAAGTATGTATGGCCGGAGCTCCCAGG
CCCTGAATGAAGAAGAGTCAGAGAGGAAGCTTCTTGATGGGCATCCAGCACCTGCGTCCCCGGCCAAAGA
GAAGATCCCTGCTATCCTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001286651
Insert Size 792 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001286651.1, NP_001273580.1
RefSeq Size 1274 bp
RefSeq ORF 792 bp
Locus ID 65963
UniProt ID Q9R1Q6
Cytogenetics 6 23.75 cM
Summary May play a role in the process of maturation of dendritic cells (By similarity). Required for the development of cerebellar granule cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1 through 6 encode the same protein.
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Documents
Resources
"NM_001286651" in other vectors (1)
SKU Description Size Price
MR228869 Tmem176b (myc-DDK-tagged) - Mouse transmembrane protein 176B (Tmem176b), transcript variant 5 10 ug
$289.00 $300.00

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