Rad51b (NM_001252562) Mouse Untagged Clone

CAT#: MC226626

Rad51b (untagged) - Mouse RAD51 homolog B (Rad51b), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad51b
• Synonyms: AI553500; mREC2; R51H2; Rad51l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226626 representing NM_001252562
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCAAGAAACTAAGACGAGTGGGTTTATCTCCAGAGCTGTGTGACCGTTTAAGCAGATACCAGA
  TTGTTAACTGTCAGCACTTTTTAAGTCTCTCCCCACTAGAACTTATGAAAGTGACTGGCCTGAGTTACAG
  AGGTGTCCACGAGCTTCTTCATACAGTAAGCAAGGCCTGTGCCCCGCAGATGCAAACGGCTTATGAGTTA
  AAGACACGAAGGTCTGCACATCTCTCACCGGCATTCCTGTCTACTACCCTGTGCGCCTTGGATGAAGCAT
  TGCACGGTGGTGTGCCTTGTGGATCTCTCACAGAGATTACAGGTCCACCAGGTTGCGGAAAAACTCAGTT
  TTGCATAATGATGAGTGTCTTAGCTACATTACCTACCAGCCTGGGAGGATTAGAAGGGGCTGTGGTCTAC
  ATCGACACAGAGTCTGCATTTACTGCTGAGAGACTGGTTGAGATTGCGGAATCTCGTTTTCCACAATATT
  TTAACACTGAGGAAAAATTGCTTCTGACCAGCAGTAGAGTTCATCTTTGCCGAGAGCTCACCTGTGAGGG
  GCTTCTACAAAGGCTTGAGTCTTTGGAGGAAGAGATCATTTCGAAAGGAGTTAAGCTTGTGATTGTTGAC
  TCCATTGCTTCTGTGGTCAGAAAGGAGTTTGACCCGAAGCTTCAAGGCAACATCAAAGAAAGGAACAAGT
  TCTTGGGCAAAGGAGCGTCCTTACTGAAGTACCTGGCAGGGGAGTTTTCAATCCCAGTTACATTTCAACC
  GATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252562
• Insert Size: 777 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252562.1, NP_001239491.1
• RefSeq Size: 1757 bp
• RefSeq ORF: 777 bp
• Locus ID: 19363
• UniProt ID: O35719
• Cytogenetics: 12 C3
• Gene Summary: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks several exons but includes an alternate 3_ terminal exon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.