Adal (NM_001290812) Mouse Untagged Clone

CAT#: MC226595

Adal (untagged) - Mouse adenosine deaminase-like (Adal), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adal
• Synonyms: 4930578F03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226595 representing NM_001290812
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATTGACAGAAGAGGTGGCCCAACAATAGCCAGGGAGACTGTCGAACTTGCTAAAGAGTTCTTCC
  TCTCTACTGAGAATACAGTTCTTGGCCTTGACCTCAGTGGAGATCCAACTATAGGACAAGCTAACGACTT
  CTTGGAGCCTCTTTTAGAAGCTAAAAAAGCAGGTCTGAAGTTGGCATTGCATCTTGCCGAGATTCCAAAC
  AGGGAAAAAGAAAATCAGATGCTGCTGAGTCTGCTCCCTGACAGAATTGGGCATGGGACATTCCTTAGCG
  CCTCTGAGGCAGGGGCCCTGGACCAAGTGGACTTCGTGAGGCAACACCAGATACCCCTCGTGTTGGAAGC
  AAATGCAGAGCTCTACATGTGCAGGGCAGGCCCTCTTCTGCTGAGGCACATCCCCACTCAGAACAAGTCC
  ACATCACAAGTAATGAACATTTTGTGGGAACTTTGTTTGACCTCAAACATCAAAAGCCAGACAGTCCCTT
  CTTATGATCAGCATCACTTTGGATTTTGGTACAGCATTGCTCATCCTTCTGTGATCTGTACTGATGATAA
  AGGTGTTTTTGCGACATACCTTTCTCAAGAATATCAACTGGCAGCAGAAACATTTAATTTGACCCCATTT
  CAGGTGTGGGATCTGTCCTATGAATCCATCAACTACATCTTTGCTTGTGACAACACCAGATCTGAACTGA
  GAAAAAGGTGGACTCACTTGAAGCAGAAAGTGTTAAACTGTAATGAGGTGAACTACTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290812
• Insert Size: 762 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290812.1, NP_001277741.1
• RefSeq Size: 2369 bp
• RefSeq ORF: 762 bp
• Locus ID: 75894
• UniProt ID: Q80SY6
• Cytogenetics: 2 E5
• Gene Summary: Putative nucleoside deaminase. May catalyze the hydrolytic deamination of adenosine or some similar substrate and play a role in purine metabolism (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains alternate 5' exon structure, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, and it also includes an additional in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to variant 1.