Mcub (NM_001293644) Mouse Untagged Clone

CAT#: MC226591

Ccdc109b (untagged) - Mouse coiled-coil domain containing 109B (Ccdc109b), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcub
• Synonyms: 9030408N13Rik; Ccdc109b; MCUb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226591 representing NM_001293644
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGAGCTCTGTCCGGCAGGAGGATGCTCCCGAGCGGGCTATGTCTGGGTCGGTGGCAGCTGCTGC
  GGACGATCCGAGCCCGCGGCCGCGGGGATCCCAGGGAGCTGCCATCCACACCCCAGGTTTTATGTATGAA
  ACTCTATGGAAATCCAAAATACCACCAAGCACTCCATTATGGGACAGTGGAGCCACAGGATGAAATAACA
  GTTACTTATAAACACGGCCTTCCCTTGGTAACCCTCACTCTGCCATCCAGAAAAGAGCGCTGCCAGTTTG
  TAGTCAAGCCGATGTTGTCGACAGTAGGCTCATTCCTGCAGGACTTACAGAATGAGGATAAAGGCATCAA
  AACTGCAGCCATCATCACGGCAGATGGCAGTGAAATTCCAGCTTCAACATTGATGGACACTTTGCTAATG
  ACAGATTTTAAGCTCATCATTAACAAACTACGGTATGATATACGGTGTCACAAGAAAGGTCAAAGCTGCA
  ATAGAAGCCCGGTCGGAAGCCAACACCAGTGGTCTCCTGTGGGCCGGCTTAGCTCTGCTCTCCGTGCAGG
  GCGGGGCGCTGGCCTGGCTTACTTGGTGGGTGTACTCCTGGGATATCATGGAGCCAGTTACCTTCTTCCT
  GTCGTTTGCGAATTCCATCGTCTTTTTTGCATACTTCATCATAACCCGGCAGAACTACACATACTCATCC
  CTCCGGAGCAGGCAGTTTCTTCAGTTCTTCCACAAGAAATCGCAGCGGCGGTGTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293644
• Insert Size: 759 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293644.1, NP_001280573.1
• RefSeq Size: 1171 bp
• RefSeq ORF: 759 bp
• Locus ID: 66815
• UniProt ID: Q810S1
• Cytogenetics: 3 G3
• Gene Summary: Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region resulting in a frameshift compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.