Ankzf1 (NM_001267620) Mouse Untagged Clone

CAT#: MC226506

Ankzf1 (untagged) - Mouse ankyrin repeat and zinc finger domain containing 1 (Ankzf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ankzf1
• Synonyms: 1300008P06Rik; 2810025E10Rik; D1Ertd161e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226506 representing NM_001267620
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACCGGCTGGCGATGCGGCAGTAGGATCTCGTTCTGGAGAGTTGTTTCTGCCCTCCGTTTCTT
  CTTCAGCCACGTCGCCAGCTCCAAGTGCAGCCTCAGCCCCTGCCTCCGTTTCCCTGTTGTCCCTCAACGG
  GGAAGCTCCACTCATTCGGGGCCTGAGCCTGGTGAGCCAAGCTCCTGGGGAGGCTCTGGCCTGGGCGCCG
  CGTACTTCCTGTCCAGGAGAGAACACAAGCTCGGGGGGAAAAGTATCTCCATATTCTCTGGAAATTTCAG
  AGAAATTATTTTGTTCAGCCTGTGACCAGATCTTCCAGAACCACCAGGAACAGAGAGAACATTATAAGCT
  TGATTGGCATCGCTTTAACTTAAAACAACGTCTCAAGAACAAGCCTCTCCTGTCTGCTTCAGACTTTGAA
  CAGCAAAGCTCCACAGGGGATCTTTCCAGCATCTCAGGATCAGATGACACAGACTCCAGCAGTGAAGAGG
  ACTTGCTGCCACTGGATGAGGGGAGGGCTGAATCCGAGAAACCTAACCGGCCCCCCGGATTCTACCCACA
  TCGGGTCCTTTTCAAAAACGCCCAGGGCCAGTTTCTTTATGCCTATCGTTGTGTCCTAGGCCCTCACCAG
  GCAAGTAACAGTCCAGATTGTGTGCTCACTCCCAACTTTTGTATACTAGGTTTTCCTCAGCCAATGTATG
  TCTTATTTTCTTCCTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267620
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267620.1, NP_001254549.1
• RefSeq Size: 1218 bp
• RefSeq ORF: 720 bp
• Locus ID: 52231
• UniProt ID: Q80UU1
• Cytogenetics: 1 38.62 cM
• Gene Summary: Plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (By similarity). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several 3' exons but includes an alternate 3' exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct and shorter C-terminus, compared to isoform 1.