Thg1l (NM_001290737) Mouse Untagged Clone
CAT#: MC226498
Thg1l (untagged) - Mouse tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) (Thg1l), transcript variant 2
"NM_001290737" in other vectors (1)
Product Images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Thg1l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC226498 representing NM_001290737
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAGCAGGTTTGCTGAGGAGCACAACTTTGCCAAACCTAATGACAGTCGGGCCCTCCATCTGATGA CCAAATGTGCCCAGACAGTAATGGAGGAGCTGGAGGACATTGTGATTGCGTACGGCCAGAGTGACGAGTA CAGCTTTGTGTTCAGGAAGAAGAGCAATTGGTTCAAAAGAAGAGCCAGTAAGTTCATGACTCTCGTGGCC TCCCAGTTTGCCTCCAGTTACGTATTTTACTGGCGGGATTACTTTGAGGACCAGCCCCTTCGGTATCCTC CAGGATTTGATGGGAGAGTCGTGTTGTATCCTAGCAACCAGACTTTGAAGGACTACCTCAGCTGGCGACA GGCAGACTGTCACATCAATAATCTTTATAATACAGTTTTCTGGGCCCTTATCCAGCAGTCTGGACTAACA CCAGTCCAAGCCCAGCAGAGATTAAAGGGAACTCTGACGGCCGACAAGAATGAGATTCTGTTCTCTGAGT TCCACATCAACTACAACAATGAGCCACACATGTACAGGAAAGGGACGGTGTTGGTGTGGCAAAAGGTGGA AGAAGTCAGAACACAAGAAGTCAGGCTGCCAGCAGAAATGGAAGGGGAAAAGAAGGCCGTGGCTCGGACC AGAACCCGGGTGGTGGCCTTGAACTGTGATCTTATTGGGGATGCCTTCTGGAAGGAACACCCAGAGATTC TGGCGGAAGAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001290737 |
Insert Size | 717 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290737.1, NP_001277666.1 |
RefSeq Size | 3740 bp |
RefSeq ORF | 717 bp |
Locus ID | 66628 |
Cytogenetics | 11 B1.1 |
Gene Summary | Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate start codon, compared to variant 1. This variant (2) has a shorter and distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR228709 | Thg1l (myc-DDK-tagged) - Mouse tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) (Thg1l), transcript variant 2 |
USD 330.00 |
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