Adora1 (NM_001291928) Mouse Untagged Clone

CAT#: MC226478

Adora1 (untagged) - Mouse adenosine A1 receptor (Adora1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adora1
• Synonyms: A1-AR; A1AR; A1R; AA1R; AI848715; BB176431; Ri
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226478 representing NM_001291928
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGCTCCTTGCCTCCGGGTCGGCGGACACCTGTGGGCACTGTGGATTCACAGGGGATCCTTGTGG
  AGTACAAGACAGTGGTGACTCAGCGGCGGGCAGCAGTGGCCATAGCCGGCTGCTGGATTCTCTCCCTTGT
  GGTAGGCCTGACACCCATGTTTGGCTGGAACAACCTGAGTGAGGTGGAACAAGCCTGGATAGCCAACGGC
  AGTGTTGGGGAGCCCGTGATCAAGTGTGAGTTCGAGAAGGTTATCAGCATGGAGTACATGGTCTACTTCA
  ACTTCTTCGTCTGGGTGCTGCCGCCATTGCTCCTCATGGTTCTTATCTACCTGGAGGTCTTCTACCTGAT
  CCGCAAGCAGCTCAACAAAAAGGTGTCAGCCTCCTCTGGTGACCCCCAGAAGTACTACGGGAAGGAGCTC
  AAGATCGCCAAGTCGCTGGCCCTCATCCTCTTCCTCTTTGCCCTCAGCTGGCTACCGCTACACATCTTGA
  ACTGCATCACCCTCTTCTGCCCCACCTGCCAGAAACCCAGCATCCTCATCTACATTGCCATCTTCCTCAC
  ACACGGCAACTCGGCCATGAACCCCATCGTCTATGCCTTCCGAATCCACAAGTTCCGGGTCACCTTTCTG
  AAGATTTGGAACGACCACTTCCGATGCCAGCCCAAGCCCCCCATTGAGGAAGATATCCCAGAGGAGAAAG
  CTGATGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291928
• Insert Size: 711 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291928.1, NP_001278857.1
• RefSeq Size: 4588 bp
• RefSeq ORF: 711 bp
• Locus ID: 11539
• UniProt ID: Q60612
• Cytogenetics: 1 E4
• Gene Summary: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 2. The encoded protein (isoform b) has a distinct N-terminus and is shorter, compared to isoform a.