Mettl21b (NM_001205036) Mouse Untagged Clone

CAT#: MC226445

LOC100504608 (untagged) - Mouse protein FAM119B-like (LOC100504608)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mettl21b
• Synonyms: eef1akmt3; Fam119b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226445 representing NM_001205036
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCTCGCACCGATCCGGAAACTGAGCCCGAATCCGTGTTCCCACGGGAGATCCGGCTCTTCA
  CAGACTCTTACTCAGAGAGCAGCCGGTTCTGCTTCTGTGGGCACGAGCTGAGCATCACGCAGAACTTTGG
  GTCGCGCCTTGGGGTGGCGGCACGCGTGTGGGATGCGGCTCTGAGCCTGTGTGACTATTTCGAGAGTCAA
  AATGTGGATTTTCGAGGCAAGAAGGTGATCGAACTGGGCGCGGGGACGGGCATCGTGGGAATCTTGGCGG
  CGCTGCAGGGGGGGGATGTTACCATCACTGATTTGCCAGTGGCACTAGAGCAAATCCAGGATAACGTCCA
  CGCCAATGTGCCACCCGGAGGCCGGGCTCGGGTGTGCGCCTTGTCCTGGGGAATTGACCAGCATGTCTTT
  CCTGGAAACTATGACTTGGTGCTGGGGGCGGATATCGTGTACCTGGAACCTACCTTCCCGCTACTGTTGG
  GGACTCTCCGACACCTGTGTGGGCCCCACGGCACCATCTACCTGGCCTCCAAGATGAGAGCAGAACACGG
  GGCAGAGACCTTCTTTCGGCGCCTCCTGCCCCAGCACTTCCACCTGGAGCTGGCCCAGCGGGATGAGGAT
  GTGAATGTCAACATCTATAGGGCCAGGCACAGGGAGGTGGCACCTGCCGGACAGCACCCCTTCTGTTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001205036
• Insert Size: 699 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205036.1, NP_001191965.1
• RefSeq Size: 699 bp
• RefSeq ORF: 699 bp
• Locus ID: 100504608
• UniProt ID: D3YWP0
• Cytogenetics: 10 D3
• Gene Summary: Protein-lysine methyltransferase that selectively methylates EEF1A1 and EEF1A2 at 'Lys-165' in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.[UniProtKB/Swiss-Prot Function]