Tlcd1 (NM_001291236) Mouse Untagged Clone

CAT#: MC226432

Tlcd1 (untagged) - Mouse TLC domain containing 1 (Tlcd1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlcd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226432 representing NM_001291236
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATGGCCGTGGGATCTTTCGCGGGGTGCTCGGACTGGGCTCCCTCTTGCTATTCCCATCACGGGTG
  AACAGACGCCAAGGATCTCACCTGATTCCGTTTGGAAAACTACCCAACCAAGAACGGCTGTGGGAAACCC
  AAGCTTATGGCAAACCCCTGAAATGCTGGTGGAGATTGAAACAGCGTGGTCAGCTTCTGGCTATTTGCTT
  GTTTGCTTCTCTGCAGGATACTTTATCCACGACACAGTGGACATTGTGGTTAGCAAGCAAACTCGAGCTT
  CTTGGGAATACCTTGTTCATCATGTCATGGCTATGGGCGCCTTCTTCTCTGGTATCTTTTGGAAAAGATT
  TGTTGGTGGTGGCGTCTTAACACTACTGGTAGAGGTCAGCAACATCTTCCTCACCTTAAGGATGATGATG
  AAGATAAACAATGCTCAGGATCTCCTCCTCTACAAGGTCAACAAGTATATCAACTTGGTCATGTACTTCC
  TCTTCCGATTGGCCCCTCAGGCCTACCTCACCAAGTTCTTCCTACAGTATGCTGGCCAGAGGACCCTGGG
  CACATTCTTGTTGGCCATCCTGCTCATGCTGGACTTGATGATAATCATCTACTTTTCTCGCCTCCTCCGC
  TCTGATTTCTGCCCTGAACGTGCACCCAGGCGCCAACAAAAAGACAAATTCTTGACTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291236
• Insert Size: 693 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291236.1, NP_001278165.1
• RefSeq Size: 1619 bp
• RefSeq ORF: 693 bp
• Locus ID: 68385
• UniProt ID: Q99JT6
• Cytogenetics: 11 B5
• Gene Summary: Regulates the composition and fluidity of the plasma membrane (By similarity). Inhibits the incorporation of membrane-fluidizing phospholipids containing omega-3 long-chain polyunsaturated fatty acids (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does not appear to have any effect on LCPUFA synthesis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice sit in the 5'-terminal exon, and uses an alternate start codon, compared to variant 1. The encoded isoform (c) has a shorter and distinct N-terminus, compared to isoform a. Both variants 3 and 4 encode the same isoform.