Csn2 (NM_001286020) Mouse Untagged Clone

CAT#: MC226430

Csn2 (untagged) - Mouse casein beta (Csn2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Csn2
• Synonyms: Csnb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226430 representing NM_001286020
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTCTTCATCCTCGCCTGCCTTGTGGCCCTTGCTCTTGCAAGAGAGACTACATTTACTGTATCCT
  CTGAGACTGATAGTATTTCCAGTGAGGAATCTGTTGAACATATCAATGAGAAACTTCAGAAGGTGAATCT
  CATGGGACAGCTGCAGGCAGAGGATGTGCTCCAGGCTAAAGTTCACTCCAGCATCCAGTCACAGCCCCAG
  GCCTTTCCATATGCTCAGGCTCAAACCATCTCTTGCAATCCCGTCCCACAAAACATCCAGCCTATTGCTC
  AACCCCCTGTGGTGCCATCTCTTGGGCCTGTCATTTCTCCTGAACTGGAATCCTTCCTTAAAGCTAAAGC
  CACCATCCTTCCCAAGCACAAACAGATGCCCCTCCTTAACTCTGAAACTGTGCTCCGCCTCATAAACTCT
  CAAATCCCCAGCCTTGCCAGTCTTGCTAATCTGCACCTTCCTCAGTCTCTGGTCCAGCTCCTGGCACAGG
  TTGTTCAGGCTTTTCCTCAGACTCACCTGGTTTCTTCTCAGACCCAGCTGTCTCTTCCTCAGTCCAAAGT
  TCTGTACTTTCTGCAGCAAGTAGCACCCTTCCTTCCACAAGATATGTCTGTCCAAGACCTTCTGCAGTAC
  CTAGAACTTCTTAACCCCACCGTCCAATTCCCTGCCACTCCACAACATTCCGTTTCTGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286020
• Insert Size: 693 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286020.1, NP_001272949.1
• RefSeq Size: 1126 bp
• RefSeq ORF: 693 bp
• Locus ID: 12991
• UniProt ID: P10598
• Cytogenetics: 5 43.56 cM
• Gene Summary: Important role in determination of the surface properties of the casein micelles.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Both variants 2 and 3 encode isoform b.