Pir (NM_001301402) Mouse Untagged Clone

CAT#: MC226361

Pir (untagged) - Mouse pirin (Pir), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pir
• Synonyms: 2310042L19Rik; Pirnl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226361 representing NM_001301402
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATGAAGACTTTTGTGGACATGTTGGTAAAATGAACCCAGGAGACCTACAGTGGATGACTGCAG
  GACGAGGCATTCTGCATGCTGAGATGCCCTGCTCAGAGGAGCCAGCTCATGGCTTGCAGCTGTGGGTGAA
  TTTGAGACGCTCAGAAAAGATGGTGGCGCCTCAGTACCAGGAACTGAAAAGTGAAGAGATCCCGAAACCC
  ACCAAAGATGGTGTGACAGTTGCTGTCATTTCAGGAGAAGCCTTGGGAATAAAGTCGAAGGTTTACACTC
  GCACACCAACCTTATATCTGGACTTCAAGTTGGACCAAGGAGCAAAGCATTCCCAGCCTATTCCTAAAGG
  GTGGACAAGCTTCATTTATACCATCTCTGGAGATGTATATATTGGGCCTGATGATGCTCAACAAAAGATA
  GAACCACATCACACAGCAGTCCTTGGAGAAGGTGACGCTGTCCAGTTGGAAAATAAGGACCCCAAAAGAA
  GCCATTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTGTCCAACATGGTCCATTTGTGATGAA
  TACCAATGAGGAAATTTCTCAGGCCATTCTTGATTTCAGAAATGCAAAAAATGGTTTTGAGGGGGCCAGA
  ACCTGGAAGTCAAAGATTGGAAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301402
• Insert Size: 657 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301402.1, NP_001288331.1
• RefSeq Size: 1472 bp
• RefSeq ORF: 657 bp
• Locus ID: 69656
• Cytogenetics: X F5
• Gene Summary: Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in translation initiation at a downstream in-frame start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.