Tle1 (NM_001285531) Mouse Untagged Clone

CAT#: MC226263

Tle1 (untagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tle1
• Synonyms: C230057C06Rik; Estm14; Grg-1; Grg1; Tle4l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226263 representing NM_001285531
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCGCAGAGCCGGCACCCAACGCCGCACCAAGCTGCAGGCCAGCCCTTTAAGTTCACTATCCCGG
  AGTCTCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGTCTTAAATTGGAGTG
  TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTACGTGATGTATTATGAAATGTCATATGGA
  TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAACACCATTTGTGCCCAAGTCATCC
  CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTGGAACGTGCCAAACAGGTGACCATGGC
  AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTCTCCCATGGCCATGGACCCCCA
  GTACCTCTCACGCCTCACCCTTCAGGACTTCAGCCTCCTGGCATCCCGCCCCTTGGGGGCAGTGCCAGCC
  TTCTCGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCATGA
  TGCAGAGCGCCACAGAGGTGAGAGGCCGGGCAAGCCAGATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285531
• Insert Size: 603 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285531.1, NP_001272460.1
• RefSeq Size: 2098 bp
• RefSeq ORF: 603 bp
• Locus ID: 21885
• UniProt ID: Q62440
• Cytogenetics: 4 C2
• Gene Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks several exons in the central and 3' coding regions, but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (d) has a distinct C-terminus and is significantly shorter than isoform a.