Ak1 (NM_001198791) Mouse Untagged Clone

CAT#: MC226208

Ak1 (untagged) - Mouse adenylate kinase 1 (Ak1), transcript variant 3


  "NM_001198791" in other vectors (1)

Reconstitution Protocol

USD 330.00

2 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ak1
Synonyms Ak-1; B430205N08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226208 representing NM_001198791
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGAAGCTGAAGAAGGCCAAGATCATCTTTGTGGTGGGCGGACCTGGCTCAGGAAAGGGCACCC
AGTGCGAGAAGATTGTACAGAAATATGGCTACACCCACCTGTCTACTGGGGACCTGCTTCGGGCAGAAGT
CAGCTCTGGATCGGAGAGGGGCAAGAAGCTGTCGGCTATCATGGAGAAGGGAGAGCTAGTGCCACTGGAC
ACGGTGCTGGACATGCTCCGAGATGCTATGTTAGCCAAAGTGGATTCTTCCAATGGCTTCCTGATCGACG
GCTACCCGAGGGAGGTGAAACAGGGAGAAGAATTTGAACAGAAGATTGGACAGCCCACACTGCTGCTGTA
TGTGGACGCAGGCGCCGAGACCATGACCCAACGACTCCTGAAGCGAGGGGAGACCAGTGGCCGCGTGGAT
GACAACGAGGAGACCATCAAGAAGCGGCTGGAGACTTATTACAATGCCACAGAACCTGTCATCTCCTTCT
ATGACAAGCGTGGCATTGTGCGCAAGGTCAATGCCGAAGGCACGGTGGACACTGTCTTCTCTGAGGTCTG
CACCTATCTTGACTCCCTGAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001198791
Insert Size 585 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001198791.1, NP_001185720.1
RefSeq Size 2088 bp
RefSeq ORF 585 bp
Locus ID 11636
UniProt ID Q9R0Y5
Cytogenetics 2 22.09 cM
Gene Summary Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs at the 5' UTR and 5' coding region, compared to variant 1. The encoded protein (isoform 2) has a shorter, distinct N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same protein (isoform 2).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.