Ing1 (NM_001302458) Mouse Untagged Clone

CAT#: MC226137

Ing1 (untagged) - Mouse inhibitor of growth family, member 1 (Ing1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ing1
• Synonyms: 2610028J21Rik; AA407184; AI875420; mING1h; p33Ing1; p37Ing1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226137 representing NM_001302458
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCTGGTGGAGAACCGCAGCAGACAGGTGGACAGTCACGTGGAGCTCTTCGAAGCACACCAGG
  ACATCAGTGACGGCACTGGTGGCAGCGGCAAGGCGGGCCAGGACAAGTCGAAGAGTGAGGCCATCACACA
  GGCAGATAAGCCGAATAACAAGCGGTCCAGGAGGCAGCGAAACAATGAGAATCGAGAGAACGCGTCGAAT
  AATCACGACCATGATGACATCACCTCAGGAACGCCCAAGGAGAAGAAAGCAAAAACCTCAAAGAAGAAGA
  AACGCTCCAAGGCCAAAGCAGAGAGGGAAGCGTCTCCTGCCGACCTTCCCATCGACCCCAACGAGCCCAC
  GTACTGTCTGTGCAACCAGGTCTCCTACGGGGAGATGATCGGCTGTGACAACGACGAATGCCCCATCGAG
  TGGTTCCACTTCTCCTGCGTGGGGCTCAACCATAAACCAAAGGGCAAGTGGTACTGCCCCAAGTGCCGTG
  GGGAGAGCGAGAAGACCATGGACAAAGCCCTGGAGAAGTCCAAGAAAGAGAGGGCTTACAACAGGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001302458
• Insert Size: 558 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302458.1, NP_001289387.1
• RefSeq Size: 2061 bp
• RefSeq ORF: 558 bp
• Locus ID: 26356
• UniProt ID: Q9QXV3
• Cytogenetics: 8 A1.1
• Gene Summary: Isoform 1 inhibits p53-dependent transcriptional activation and may function as an oncoprotein. Isoform 2 acts as a negative growth regulator by cooperating with p53 in transcriptional activation of p53-responsive genes and may act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an upstream alternate 5' terminal exon and lacks an exon in the 5' region, which results in the use of a downstream start codon, compared to variant 1. It encodes isoform 3, which has a shorter N-terminus, compared to isoform 1. Variant 3, 4, 5 and 6 encode the same protein.