Srsf10 (NM_001284196) Mouse Untagged Clone

CAT#: MC226126

Srsf10 (untagged) - Mouse serine/arginine-rich splicing factor 10 (Srsf10), transcript variant 4


  "NM_001284196" in other vectors (1)

Reconstitution Protocol

USD 330.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Other products for "Srsf10"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Srsf10
Synonyms Fusip1; FUSIP2; Nssr; NSSR1; NSSR2; Sfrs13a; SRrp40; Srsf13a; TASR; TASR1; TASR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC226126 representing NM_001284196
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGATACCTGCGCCCCCCTAACACGTCTCTGTTCGTCAGGAACGTGGCGGACGACACCAGGTCTG
AAGATTTACGTCGGGAATTTGGTCGTTATGGTCCAATAGTAGATGTTTATGTCCCACTTGATTTCTACAC
TCGGCGTCCAAGAGGATTTGCATATGTTCAATTTGAGGATGTTCGTGATGCTGAAGACGCTTTACATAAT
TTGGACAGAAAATGGATTTGTGGGCGTCAGATTGAAATCCAGTTCGCACAGGGGGATCGGAAGACACCAA
ATCAAATGAAAGCCAAGGAAGGGAGGAATGTATACAGCTCTTCACGATATGACGATTATGACCGATATAG
ACGCTCTCGAAGCCGGAGTTATGAAAGGAGAAGATCGAGGAGTCGCTCCTTTGATTATAACTATAGGAGA
TCTTACAGTCCTAGAAATAGACCGACTGGAAGACCACGGCGTAGCCGAAGCCATTCCGACAATGATAGAC
CAAACTGCAGCTGGAATACCCAGTACAGTTCTGCTTACTACACTTCAAGAAAGATCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001284196
Insert Size 549 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001284196.1, NP_001271125.1
RefSeq Size 3433 bp
RefSeq ORF 549 bp
Locus ID 14105
UniProt ID Q9R0U0
Cytogenetics 4 D3
Gene Summary Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (By similarity). May be involved in regulation of alternative splicing in neurons (PubMed:10583508).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region and contains an alternate 3' terminal exon, compared to variant 2. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 2.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.